APC Rabbit anti-Human/Monkey CD127/IL-7R mAb for FC - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7R Function and Localization

• CD127/IL-7R alpha is a transmembrane protein that serves as the alpha subunit of the interleukin-7 receptor, encoded by the IL7R gene in humans (Gene ID 3575).
• It functions as a receptor for interleukin-7 (IL-7), a cytokine critical for lymphocyte development and homeostasis.
• Additionally, CD127 acts as a receptor for thymic stromal lymphopoietin (TSLP), linking it to type 2 immune responses.
• The protein is primarily located on the cell membrane, but a secreted splice variant (sIL-7R) exists due to alternative splicing, as indicated by UniProt keywords.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination (Ubl conjugation), which regulate its stability and signaling.
• Mutations in IL7R are associated with severe combined immunodeficiency (SCID), highlighting its essential role in immune function.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 21-236), suggesting the antibody recognizes the native conformation on live cells; consider using flow cytometry with appropriate live/dead discrimination.
• For flow cytometric applications, titrate the antibody to determine optimal concentration and use relevant isotype controls for accurate gating.
• Validate cross-reactivity in your specific sample system, such as human peripheral blood mononuclear cells (PBMCs) or cynomolgus monkey (Macaca fascicularis) tissues.
• To distinguish membrane-bound CD127 from soluble isoforms, additional assay formats (e.g., ELISA, Western blot) may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to high-quality IVD raw materials. For targets like CD127/IL-7R, our portfolio includes validated monoclonal antibodies, antibody pairs, and ancillary reagents suitable for immunoassay development and flow cytometric applications. We also support custom sourcing requests for rare or challenging targets, ensuring a reliable supply chain from concept to clinic.