APC Rabbit anti-Human Siglec-8 Monoclonal Antibody for FC - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8, also known as SAF-2 or SIGLEC8L, is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
• It functions as a putative adhesion molecule that mediates sialic-acid-dependent binding to blood cells. Related references: PMID:10625619, PMID:10856141
• Preferentially binds to α-2,3-linked sialic acid and also to α-2,6-linked sialic acid; the binding site may be masked by cis interactions on the same cell surface. Related references: PMID:10625619
• Recognizes glycans with terminal N-acetylneuraminic acid and underlying 6-O-sulfated galactose, with a preference for 6-sulfated sialyl-Lewis X epitopes. Related references: PMID:27357658
• The protein is a type I transmembrane glycoprotein localized to the cell membrane.
• Expressed specifically on the surface of basophils, mast cells, and eosinophils.
• Contains immunoglobulin-like domains, disulfide bonds, and undergoes N-linked glycosylation (keywords: Glycoprotein, Disulfide bond, Repeat, Signal, Transmembrane).
• Alternative splicing generates multiple isoforms (keyword: Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 17-363), encompassing the sialic acid-binding domains, making it suitable for detecting surface-expressed Siglec-8 in flow cytometry.
• For FC, titrate the antibody to determine optimal working concentration; use appropriate isotype controls and consider cell fixation/permeabilization conditions if needed.
• Reported cross-reactivity is limited to human; validate in other species if required for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive sourcing solution for IVD raw materials. This APC-conjugated anti-Siglec-8 monoclonal antibody supports flow cytometry applications in allergy and immunology research. Beyond this product, CamelBio offers validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials to accelerate assay development from concept to clinic.