APC Rabbit anti-Human Podoplanin Monoclonal Antibody for Flow Cytometry - Q86YL7

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin (PDPN), also known as Aggrus, Gp36, T1-alpha, and PA2.26 antigen, is a type I transmembrane sialoglycoprotein encoded by the PDPN gene.
• It mediates cell adhesion and migration by interacting with partners such as CLEC1B, CD9, MSN, EZR, and CD44, playing critical roles in blood/lymphatic vessel separation, platelet aggregation, and epithelial-mesenchymal transition (EMT). Related references: PMID:14522983 PMID:15231832 PMID:17222411 PMID:18541721 PMID:17046996 PMID:21376833 PMID:20962267
• Podoplanin promotes tumor cell invasion by controlling invadopodia stability and ECM degradation through RHOC-ROCK-LIMK-CFL1 signaling. Related references: PMID:25486435
• It is localized to the plasma membrane and various cell projections including lamellipodia, filopodia, microvilli, ruffles, and invadopodia; also found in membrane rafts and cytosol.
• Post-translational modifications include extensive O-glycosylation and sialylation; alternative splicing generates multiple isoforms.
• Highly expressed in placenta, lung, skeletal muscle, and brain; weak expression in kidney and liver; upregulated in colorectal tumors and a subset of oral squamous cell carcinomas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-123) of human podoplanin, ensuring recognition of native membrane-bound PDPN.
• This APC-conjugated antibody is designed for flow cytometry; consider titrating for optimal staining signal-to-background ratio.
• For cell adhesion/migration functional studies, validate the antibody's specificity in the relevant cell system (e.g., tumor cells or activated platelets).
• Since podoplanin is highly glycosylated, treatment with deglycosylation enzymes may alter the detection signal if the epitope is carbohydrate-dependent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This APC-conjugated anti-podoplanin mAb supports cancer and cell biology research. Contact us to streamline your procurement and development needs.