APC Rabbit anti-Human NT5E/CD73 Monoclonal Antibody for Flow Cytometry - P21589

Core Product Specifications and Parameters

Biological Background: NT5E/CD73 Function and Localization

• Core enzymatic activity: NT5E/CD73 is a 5'-nucleotidase that hydrolyzes extracellular nucleoside monophosphates into nucleosides and inorganic phosphate, with a strong preference for AMP over other substrates. This step is rate-limiting in the generation of immunosuppressive adenosine from ATP.
  Related references:
  PMID:21933152 PMID:22997138 PMID:23142347 PMID:34403084
• Substrate specificity: Besides AMP, the enzyme can also act on IMP, UMP, GMP, CMP, and several deoxynucleotides (dAMP, dCMP, dTMP), as well as NAD and NMN, though ribonucleotide monophosphates are the preferred substrates.
• Subcellular localization: CD73 is attached to the outer leaflet of the plasma membrane via a C-terminal glycosylphosphatidylinositol (GPI) anchor, positioning its active site in the extracellular space to regulate pericellular nucleotide pools.
• Post-translational modifications: The protein is N-glycosylated and contains structurally important disulfide bonds. Its membrane attachment depends entirely on the GPI anchor.
• Structural features: It belongs to the 5'-nucleotidase family and requires zinc and other divalent metal ions for catalytic activity. The molecule exists as a homodimer on the cell surface.
• Disease relevance: Mutations in NT5E are associated with arterial calcification due to deficiency of CD73 (ACDC), a rare genetic disorder, and the protein is a prominent immune checkpoint target in oncology.

Experimental Guidance and Technical Tips

• The immunogen covers the entire ectodomain (amino acids 27–547), which includes the catalytic and substrate-binding regions. This design favors recognition of native, properly folded CD73 on live cells.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your cell type of interest. Include appropriate isotype controls and viability dyes.
• CD73 is shed or released on extracellular vesicles in some systems; ensure sample preparation minimizes cell damage to avoid false-negative signals.
• This APC conjugate is well-suited for multiplex panels; verify compensation settings when combining with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a complete portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For immuno-oncology targets like CD73, we offer customized reagent sourcing and technical consulting to streamline assay development from concept to clinic. Contact our team to discuss how we can meet your specific CD73 antibody needs and scale your production securely.