APC Rabbit anti-Human Neuropilin-2/NRP2 Monoclonal Antibody for FC - O60462

Core Product Specifications and Parameters

Biological Background: Neuropilin-2 Function and Localization

• Neuropilin-2 (NRP2) is a transmembrane receptor that binds class 3 semaphorins (SEMA3C, SEMA3F) and specific isoforms of vascular endothelial growth factor (VEGF-165, VEGF-145) and placental growth factor (PLGF-2), mediating axon guidance and angiogenesis.
• It also functions as an entry receptor for human cytomegalovirus (HCMV) in epithelial and endothelial cells via a pentamer-dependent mechanism.
• Alternative splicing generates multiple isoforms, which may have distinct ligand-binding properties and biological functions.
• NRP2 is predominantly localized at the cell membrane, but a secreted isoform exists that can modulate ligand availability.
• Post-translational modifications include glycosylation, disulfide bond formation, and heparin binding, which influence ligand interactions and protein stability.
• The protein contains calcium-binding domains and is recognized as a developmental protein involved in neurogenesis, differentiation, and host-virus interaction pathways.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering the full extracellular domain (aa 1-855), suggesting it may recognize both membrane-bound and soluble NRP2 forms; consider this when designing experiments.
• For flow cytometry, include appropriate isotype controls and validate staining in physiologically relevant cell systems, as NRP2 expression levels can vary.
• The APC conjugate provides strong fluorescence; titrate the antibody to achieve optimal signal-to-noise ratio in your assay.
• NRP2 expression can be upregulated under certain conditions (e.g., inflammation, hypoxia); confirm expression profiles using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For Neuropilin-2/NRP2—a target central to angiogenesis, neurodevelopment, and viral entry—our APC-conjugated monoclonal antibody supports flow cytometry applications. We also provide rare target raw-material sourcing and technical consulting to accelerate your assay development from concept to clinic.