APC Rabbit anti-Human Nectin-1/CD111 Monoclonal Antibody for Flow Cytometry - Q15223

Core Product Specifications and Parameters

Biological Background: Nectin-1 Function and Localization

• Nectin-1 (also known as PVRL1, HveC, CD111) is a cell adhesion molecule of the immunoglobulin superfamily that mediates homophilic and heterophilic trans-interactions with nectin-3 and nectin-4.
• Promotes cell-cell contacts essential for nervous system development, including axon guidance, synaptogenesis, and neurite outgrowth. Related references: PMID:21325282
• In the auditory epithelium, heterophilic interaction with nectin-3 contributes to the checkerboard-like cellular patterning of hair cells and supporting cells.
• Required for enamel mineralization.
• (Microbial infection) Acts as a major receptor for herpes simplex virus 1 (HSV-1), HSV-2, and pseudorabies virus, mediating viral entry into host cells. Related references: PMID:21980294 PMID:25231300 PMID:28381567 PMID:34587223
• Localizes to the cell membrane, cell junctions (adherens junctions), presynaptic membranes, and is secreted.
• Multiple isoforms produced by alternative splicing exist with predicted molecular weights around 57, 51, and 39 kDa; undergoes glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (amino acids 1-334), which includes the immunoglobulin domains critical for ligand binding and viral entry; this design may favor detection of surface-expressed receptor.
• This APC conjugate is intended for flow cytometry (FC); ensure appropriate isotype controls and titrations when analyzing Nectin-1 expression on human cells.
• Given the reported molecular weight variants (57, 51, 39 kDa) from alternative splicing, confirm the relevant isoform in your target cell type if using other assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with validated antibodies, antigen reagents, and technical support from concept to clinic. Our portfolio includes optimized monoclonal antibodies like this anti-Nectin-1 (CD111) reagent for neuroscience, oncology, and infectious disease applications, along with bulk ancillary reagents and custom sourcing for rare targets. Let CamelBio streamline your raw-material procurement to accelerate assay development and commercialization.