APC Rabbit anti-Human Integrin-β1/CD29 Monoclonal Antibody for Flow Cytometry - P05556

Core Product Specifications and Parameters

Biological Background: Integrin-β1 Function and Localization

• Integrin beta-1 (ITGB1), also known as CD29, fibronectin receptor subunit beta, and VLA-4 subunit beta, is a transmembrane receptor that heterodimerizes with multiple alpha integrin subunits to mediate cell-extracellular matrix adhesion and bidirectional signal transduction.
• As the common beta subunit, ITGB1 pairs with alpha-1 through alpha-11 and alpha-V integrins to form receptors for collagen, fibronectin, laminin, fibrinogen, vitronectin, and other ligands, thereby regulating cell migration, matrix assembly, and tissue organization.
• ITGA4:ITGB1 binds to the chemokine fractalkine (CX3CL1) and acts as a coreceptor for CX3CR1-dependent fractalkine signaling, influencing leukocyte trafficking and inflammation. Related references: PMID:23125415 PMID:24789099
• ITGA5:ITGB1 serves as a receptor for fibrillin-1 and fibronectin, mediating RGD-dependent cell adhesion critical for extracellular matrix integrity and mechanosensing. Related references: PMID:12807887 PMID:17158881 PMID:33962943
• ITGA5:ITGB1 is also a functional receptor for interleukin-1 beta (IL1B), with binding required for IL1B-mediated signaling, bridging integrin function and innate immunity. Related references: PMID:29030430
• ITGB1 is targeted by several pathogens: integrin ITGA2:ITGB1 acts as a receptor for human echoviruses, rotavirus, and human parvovirus B19, facilitating viral entry. Additionally, ITGB1 interacts with fungal CotH proteins during mucormycosis. Related references: PMID:32487760
• Subcellularly, ITGB1 localizes to the plasma membrane, focal adhesions, lamellipodia, and recycling endosomes, and is detected in invadopodia and cleavage furrows, consistent with its roles in adhesion, migration, and cytokinesis.
• ITGB1 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its stability, trafficking, and signaling functions.
• Tissue expression: ITGB1 is widely expressed, with reported detection in placenta, skin, liver, muscle, and various cultured cell lines; an isoform specifically expressed in striated muscle (isoform 1D) has been identified. Related references: PMID:33962943 PMID:1551917 PMID:7545396 PMID:7681433

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-728, encompassing nearly the entire extracellular domain of human ITGB1; thus the antibody likely recognizes native conformational epitopes. For flow cytometry, consider using live, non-permeabilized cells to preserve surface antigen integrity.
• Since the antibody is APC-conjugated, protect from light and avoid freeze-thaw cycles. Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions to minimize background.
• The product is validated for flow cytometry (FC) only; for other applications like Western blot or immunohistochemistry, cross-validate performance in the relevant sample system as reactivity may differ.
• Confirm species cross-reactivity if working with non-human samples; while stated cross-reactivity is human, sequence homology suggests potential reactivity with other species, but empirical testing is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This APC-conjugated anti-Integrin-β1 antibody supports research in cell adhesion, migration, and receptor signaling, and is part of our comprehensive catalog for diagnostic development. Explore our custom sourcing capabilities for rare targets and seamless technical support from concept to clinic.