APC Rabbit anti-Human IL-2 mAb - P60568

Core Product Specifications and Parameters

Biological Background: IL-2 Function and Localization

• IL-2 is a cytokine predominantly secreted by activated CD4+ helper T-cells, serving as a potent T-cell growth factor.
  Related references: PMID:6438535
• It binds to the high-affinity trimeric IL-2 receptor (IL2RA, IL2RB, IL2RG) or low-affinity dimer (IL2RB, IL2RG), inducing receptor oligomerization.
  Related references: PMID:16293754, PMID:16477002
• Downstream signaling involves JAK1/JAK3 phosphorylation, STAT5 recruitment, and activation of PI3K and MAPK pathways.
  Related references: PMID:7973659, PMID:8580378, PMID:25142963
• IL-2 promotes B-cell proliferation and immunoglobulin production, and enhances NK-cell cytotoxicity.
  Related references: PMID:6608729, PMID:6438535
• It maintains immune tolerance by supporting regulatory T-cell survival and governs differentiation of Th1, Th2, Th17, and memory CD8+ T-cells.
• IL-2 is a secreted glycoprotein (approx. 15-18 kDa) with essential disulfide bonds for structural integrity.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry (FC), pre-treat cells with a secretion inhibitor like brefeldin A to retain IL-2 in the Golgi, and optimize fixation/permeabilization.
• The immunogen covers amino acids 21–153, corresponding to the mature human IL-2 sequence; the antibody likely recognizes both native and recombinant IL-2.
• As a rabbit monoclonal, it offers high specificity and lot-to-lot consistency; validate in stimulated human PBMCs or relevant cell lines.
• Since IL-2 is a secreted cytokine, ensure appropriate controls and consider using stimulated vs. unstimulated samples for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers with validated antibodies, bulk reagents, and rare target sourcing. For anti-IL-2 development, we can supply optimized antibody pairs, conjugated monoclonals, and ancillary reagents to streamline assay development. Whether for flow cytometry-based immune monitoring or functional studies, our one-stop platform bridges your concept to clinic needs.