APC Rabbit anti-Human IL-17A Monoclonal Antibody for Flow Cytometry (Intracellular) - Q16552

Core Product Specifications and Parameters

Biological Background: IL-17A Function and Localization

• Interleukin-17A (IL-17A), also known as CTLA-8, is a secreted glycoprotein belonging to the IL-17 cytokine family, with a molecular weight of approximately 17.5 kDa (UniProt: Q16552).
• IL-17A functions as a key effector cytokine of innate and adaptive immunity, critically involved in antimicrobial host defense and maintenance of tissue integrity. Related references: PMID:24120361
• It mediates its effects through the IL17RA-IL17RC heterodimeric receptor complex, leading to TRAF6-dependent activation of NF-kB and MAP kinase pathways and broad transcriptional induction of pro-inflammatory mediators. Related references: PMID:17911633 PMID:18684971 PMID:19825828
• As the signature cytokine of Th17 cells, IL-17A bridges adaptive and innate immunity, primarily driving neutrophil recruitment and activation at sites of infection and inflammation (By similarity).
• In mucosal surfaces, IL-17A induces antimicrobial peptides such as beta-defensins and chemokines, thereby limiting microbial entry through epithelial barriers (By similarity).
• Upon acute epithelial injury, IL-17A directly promotes barrier repair by regulating tight junction proteins including OCLN. Related references: PMID:21350122
• IL-17A is also implicated in B cell homeostasis, germinal center formation, and antiviral immune responses against West Nile virus and influenza (By similarity).
• At the protein level, IL-17A is expressed in memory Th17 cells and is secreted into the extracellular space to exert its paracrine and systemic effects.

Experimental Guidance and Technical Tips

• The antibody was generated using an immunogen spanning amino acids 24-155 of human IL-17A (mature protein). It is expected to recognize the native secreted form.
• For intracellular flow cytometry (FC intra), ensure proper fixation and permeabilization steps to allow antibody access to cytoplasmic IL-17A. Consider using standard protocols with paraformaldehyde fixation and saponin or methanol permeabilization.
• As an APC conjugate, protect the antibody from prolonged exposure to light to prevent photobleaching. Titration experiments are recommended to determine the optimal concentration for your samples.
• Reported protein weight is 18 kDa; however, glycosylation may result in slightly higher molecular weight bands. Validate specificity in your system using appropriate controls (e.g., stimulated vs. unstimulated T cells).
• This antibody is validated for human reactivity; cross-reactivity with other species has not been tested. If applied to non-human samples, verify cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For IL-17A and other immune targets, we can provide validated antibody pairs, optimized monoclonal or polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our sourcing capabilities extend to rare or custom targets, ensuring you obtain the right raw materials for your projects. Contact us to learn more about our one-stop procurement services.