APC Rabbit anti-Human IgG (Fc) Monoclonal Antibody for FC - P01857

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Gamma 1 (IGHG1) Function and Localization

• IGHG1 encodes the constant region of immunoglobulin gamma-1 (IgG1) heavy chains, which form the backbone of antibodies and serve as the B cell receptor (BCR) in the recognition phase of humoral immunity. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The Fc region of IgG1 binds tightly to specific Fc gamma receptors (FcγRs) on innate immune cells and the C1q component of complement, triggering antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and complement-dependent cytotoxicity (CDC).
• IGHG1 is located on chromosome 14 and is a member of a cluster of immunoglobulin heavy chain constant genes; alternative splicing and chromosomal rearrangement (class-switch recombination) enable production of different Ig isotypes.
• The protein exists in two forms: a secreted form that circulates in blood and tissues, and a membrane-bound form on B cells where it serves as an antigen receptor.
• Post-translational modifications include N-linked glycosylation in the CH2 domain critical for stability and FcγR binding, and interchain disulfide bonds that assemble heavy and light chains.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 100–330 of human IgG Fc) covers the hinge, CH2, and CH3 domains; thus the antibody likely recognizes the native Fc conformation on both soluble IgG and membrane-bound BCR.
• For flow cytometry, consider titrating the antibody to determine the optimal staining index, using appropriate isotype controls, and validating in human peripheral blood B cells or IgG1-expressing cell lines.
• The APC fluorophore is compatible with common multicolor panels (e.g., FITC, PE) but requires protection from prolonged light exposure to prevent signal degradation.
• As a rabbit monoclonal antibody, it offers high batch-to-batch consistency; for applications other than FC, additional validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with complete IgG detection solutions, including validated anti-human IgG Fc antibodies, paired reagents for antibody titer assays, and custom monoclonal antibody development. From optimized antibodies to bulk ancillary reagents, we streamline IVD raw material procurement backed by technical guidance from concept to clinic.