APC Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry (FC) - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1, also known as CD54, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It mediates cell adhesion by serving as a receptor for integrins LFA-1 (ITGAL:ITGB2) and Mac-1 (ITGAM:ITGB2), essential for leukocyte-endothelial interactions, neutrophil adhesion, and calibrating T-cell killing strength. Related references: PMID:3086451, PMID:3340213, PMID:38195629, PMID:1980124
• During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Related references: PMID:17875742
• ICAM-1 promotes cell aggregation in epithelial cells through interaction with MUC1. Related references: PMID:11173916
• Acts as a receptor for major group rhinoviruses and Coxsackievirus A21, facilitating viral entry.
• Upon Kaposi's sarcoma-associated herpesvirus infection, ICAM-1 is degraded by viral E3 ubiquitin ligase MIR2, potentially preventing lysis of infected cells.
• Predominantly localizes to the cell membrane.
• Expressed on non-hematopoietic cells (vascular endothelial cells, thymic epithelial cells, fibroblasts) and hematopoietic cells (tissue macrophages, stimulated T lymphocyte blasts, dendritic cells). Lowly expressed on peripheral blood leukocytes. Related references: PMID:3086451
• Post-translationally modified by N-glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to allophycocyanin (APC), making it convenient for flow cytometry without secondary detection steps.
• The immunogen spans the extracellular domain (amino acids 28–480), suggesting recognition of an epitope accessible on live cells.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Validate staining in human cell lines known to express ICAM-1, such as HUVECs, HeLa, or Jurkat, and include appropriate isotype controls.
• For functional studies involving viral receptors, use relevant rhinovirus or Coxsackievirus A21 strains and confirm ICAM-1 expression by parallel staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-ICAM-1 antibody addresses research needs in immunology, inflammation, and virology. Contact us to source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw materials for your next project.