APC Rabbit anti-Human Flt3/CD135 Monoclonal Antibody for FC - P36888

Core Product Specifications and Parameters

Biological Background: Flt3/CD135 Function and Localization

• FLT3 (Fms-like tyrosine kinase 3), also known as CD135, FLK2, or STK1, is a receptor-type tyrosine-protein kinase encoded by the FLT3 gene (GeneID: 2322).
• It acts as the cell-surface receptor for the cytokine FLT3LG, regulating differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells.
• Upon ligand binding, FLT3 activates multiple downstream signaling cascades, including RAS-MAPK, PI3K-AKT-mTOR, and STAT5 pathways; wild-type FLT3 only marginally activates STAT5.
• FLT3 is predominantly expressed in hematopoietic tissues: detected in bone marrow, hematopoietic stem cells, myeloid progenitor cells, and granulocyte/macrophage progenitor cells, with lower levels in kidney and pancreas.
• It is highly expressed in T-cell leukemia, underscoring its role as a proto-oncogene; constitutively activating mutations (e.g., internal tandem duplications) are common in acute myeloid leukemia (AML).
• The protein is a single-pass transmembrane glycoprotein localized to the cell membrane and endoplasmic reticulum lumen, with a molecular weight of approximately 113 kDa (UniProt) or 108-113 kDa observed.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and ubiquitination; its extracellular region contains five immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning the N-terminal extracellular domain (amino acids 27–163), making it suitable for detecting surface-expressed FLT3.
• This APC-conjugated monoclonal antibody is validated for flow cytometry (FC); consider titrating the antibody and including appropriate isotype controls for optimal staining.
• For FC analysis, humane peripheral blood, bone marrow, or leukemia cell lines (e.g., MV4-11, OCI-AML3) may serve as positive controls; validate specificity in the relevant sample system.
• Note that the predicted molecular weight of FLT3 is ~113 kDa, but glycosylation can lead to a slightly higher apparent mass; this antibody is not intended for western blotting unless specifically validated.

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CamelBio offers streamlined access to IVD raw materials, including this anti-Flt3/CD135 monoclonal antibody, alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you are developing flow cytometry panels for hematologic malignancy diagnostics or need consistent raw material supply for leukemia research kits, CamelBio serves as your Procurement and technical bridge from concept to clinic.