APC Rabbit Anti-Human EGFR Monoclonal Antibody for Flow Cytometry - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase that, upon binding EGF family ligands, undergoes homo- or heterodimerization and autophosphorylation, activating key downstream signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STATs (PubMed:27153536, PubMed:2790960). Related references: PMID:27153536, PMID:2790960
• Known EGFR ligands encompass EGF, TGF-α, amphiregulin (AREG), epigen (EPGN), betacellulin (BTC), epiregulin (EREG), and heparin-binding EGF-like growth factor (HBEGF), each triggering distinct signaling dynamics (PubMed:12297049, PubMed:15611079, PubMed:17909029). Related references: PMID:12297049, PMID:15611079, PMID:17909029
• Phosphorylated EGFR recruits adaptor proteins such as GRB2, initiating complex intracellular signaling that governs cell proliferation, survival, migration, and differentiation (PubMed:10805725). Related references: PMID:10805725
• Beyond canonical pathways, EGFR directly phosphorylates substrates like RGS16 (coupling to G protein signaling) (PubMed:11602604) and MUC1 (promoting SRC/β-catenin interactions) (PubMed:11483589). Related references: PMID:11602604, PMID:11483589
• EGFR modulates cell motility via interaction with CCDC88A/GIV, which retains the receptor at the plasma membrane to sustain pro-migratory signaling (PubMed:20462955). Related references: PMID:20462955
• It may also activate the NF-κB cascade (PubMed:11116146) and contributes to neurological processes such as learning, memory, and pain hypersensitivity (By similarity). Related references: PMID:11116146
• Isoform 2 acts as a potential antagonist of EGF action and is notably expressed in ovarian cancers.
• Subcellular localization spans the plasma membrane, endoplasmic reticulum, Golgi, endosomes, and nucleus; the receptor can also be secreted. Post‑translational modifications include glycosylation, phosphorylation, ubiquitination, and palmitoylation.
• EGFR serves as a host cell receptor for hepatitis C virus (HCV) entry in hepatocytes, facilitating CD81-CLDN1 complex formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 335–525 within the extracellular domain of human EGFR, making this APC‑conjugated antibody well‑suited for detecting surface EGFR by direct flow cytometry.
• Because EGFR is ubiquitously expressed, include appropriate isotype controls and consider using a known EGFR‑positive cell line (e.g., A431 or HeLa) for initial setup.
• For optimal signal, titrate the antibody to determine the best staining concentration and validate under your specific experimental conditions.
• If co‑staining with other fluorophores, verify spectral compatibility and perform compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, connecting diagnostic manufacturers and labs with high‑quality reagents from concept to clinic. For EGFR‑targeted research, we offer validated monoclonal antibodies, recombinant proteins, and ancillary reagents to support oncological and signaling workflow development. Let CamelBio be your trusted partner in sourcing rare‑target raw materials and optimizing your diagnostic pipeline.