APC Rabbit anti-Human CD95/FAS Monoclonal Antibody for Flow Cytometry - P25445

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• CD95/FAS (TNFRSF6) is a member of the tumor necrosis factor receptor superfamily, also known as Apo-1 antigen or FASLG receptor.
• It functions as the cell-surface receptor for TNFSF6/FASLG, transmitting apoptotic signals upon ligand binding.
• Ligand-induced trimerization recruits the adaptor FADD and procaspase-8, forming the death-inducing signaling complex (DISC), which activates the caspase cascade leading to apoptosis.
• FAS-mediated apoptosis plays a critical role in peripheral immune tolerance and activation-induced cell death of mature T-cells.
• Alternatively spliced secreted isoforms (isoforms 2–6) can act as decoy receptors, blocking apoptosis in vitro.
• The protein localizes primarily to the cell membrane and membrane rafts; soluble isoforms are also secreted.
• Isoform 1 and isoform 6 are expressed at comparable levels in resting peripheral blood mononuclear cells; upon activation, isoform 1 levels increase while isoform 6 decreases.
• Post-translational modifications include N-linked glycosylation, palmitoylation, phosphorylation, and disulfide bond formation, with an apparent molecular weight of ~38 kDa (glycosylated; theoretical 37.7 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 26–173), ensuring recognition of the native cell-surface receptor.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions, using appropriate isotype controls.
• CD95/FAS is expressed on various immune cells, including activated T and B lymphocytes; verify expression in your target population through pilot experiments.
• Since this antibody is APC-conjugated, avoid exposure to light and ensure the flow cytometer is properly compensated for the APC channel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibodies, recombinant proteins, and ancillary reagents. For apoptosis and immune tolerance research, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to your workflow needs. Whether you require rare-target raw materials or established immunoreagents, we facilitate one-stop access to support your development from concept to clinic.