APC Rabbit anti-Human CD9 Monoclonal Antibody for FC - P21926

Core Product Specifications and Parameters

Biological Background: CD9 Function and Localization

• CD9, also known as MIC3, MRP-1, TSPAN29, or p24, is a member of the tetraspanin superfamily, characterized by four transmembrane domains and two extracellular loops. It is encoded by the CD9 gene on human chromosome 12.
• CD9 functions as an integral membrane protein that associates with integrins, regulating crucial processes such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Related references: PMID:14575715, PMID:18541721, PMID:8478605
• In myoblasts, CD9 cooperates with CD81 and PTGFRN to inhibit myotube fusion during muscle regeneration (By similarity). In macrophages, it associates with CD81 and beta-1/beta-2 integrins to prevent macrophage fusion into multinucleated giant cells (PubMed:12796480). Related references: PMID:12796480
• CD9 is implicated in tumor metastasis and cell motility; it modulates cell adhesion and migration, with evidence suggesting roles in suppressing or promoting metastasis depending on the cellular context (PubMed:7511626, PubMed:8478605). Related references: PMID:7511626, PMID:8478605
• Subcellularly, CD9 localizes primarily to the cell membrane and is also detected in secreted extracellular exosomes, consistent with its trafficking and functional roles in intercellular communication.
• The protein undergoes post-translational modifications including palmitoylation and N-linked glycosylation, which are important for its membrane organization and interactions. It also contains disulfide bonds stabilizing its extracellular domains.
• CD9 is abundantly expressed on the surface of platelets and is detected in a variety of hematopoietic and epithelial cells, reflecting its broad functional involvement in blood and tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-228, encompassing the large extracellular loop of CD9. This design likely presents the native conformational epitopes, making the antibody suitable for detecting cell surface CD9 by flow cytometry.
• When performing FC, consider using known CD9-expressing cells (e.g., platelets, hematopoietic cell lines) as positive controls to verify staining patterns and gate settings.
• As the antibody is provided in an APC-conjugated format, ensure appropriate compensation controls are included in multicolor panels, especially when co-staining with other fluorophores.
• Cross-reactivity with non-human species has not been specified; if your study requires species cross-reactivity, validate independently with the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive selection of IVD raw materials, including this APC-conjugated anti-CD9 monoclonal antibody for flow cytometry applications. Our portfolio supports research in cell adhesion, tumor metastasis, and platelet biology, with validated monoclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets. Partner with CamelBio for seamless access to the critical materials you need from concept to clinic.