APC Rabbit anti-Human CD66a/CEACAM1 Monoclonal Antibody for Flow Cytometry - P13688

Core Product Specifications and Parameters

Biological Background: CD66a/CEACAM1 Function and Localization

• CD66a/CEACAM1, also known as Biliary glycoprotein 1 (BGP-1), is a member of the carcinoembryonic antigen family and mediates calcium-independent homophilic cell adhesion, contributing to tissue architecture and intercellular interactions.
• Acts as a coinhibitory receptor in immune cells: upon TCR stimulation in T cells, it recruits PTPN6 to dephosphorylate CD247 and ZAP70, inhibiting cytotoxicity and cytokine production; in NK cells, trans-homophilic interactions with target cells lead to PTPN6 recruitment and VAV1 dephosphorylation, suppressing cytolysis; thus regulating autoimmunity and anti-tumor immunity (PubMed:18424730, 23696226, 25363763).
  Related references:
  PMID:18424730
  PMID:23696226
  PMID:25363763
• Negatively regulates neutrophil activation by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, reducing inflammasome activity and IL1B production (By similarity).
• Promotes angiogenesis and arteriogenesis by facilitating endothelial cell migration and vascular remodeling, and increases collateral vessel caliber post-ischemia (By similarity).
• Modulates insulin signaling by enhancing insulin clearance and suppressing fatty acid synthesis via FASN interaction upon INSR-mediated phosphorylation (By similarity).
• Interacts with FLNA to inhibit cell migration and scattering, with this interaction disrupting FLNA-RALA binding (PubMed:16291724).
  Related references:
  PMID:16291724
• Localizes to multiple membrane domains including lateral, apical, and basal cell surfaces, adherens junctions, and microvilli; also found in secretory vesicles and secreted forms.
• Expressed in colon epithelial cells, and in immune cells such as T cells (long cytoplasmic domain forms), granulocytes, and lymphocytes; alternative splicing generates multiple isoforms with distinct functions.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration that yields maximal separation between positive and negative populations while minimizing background.
• Validate staining on known CEACAM1-expressing cells (e.g., T cells, NK cells, colon epithelial cell lines) and include appropriate isotype controls.
• Because CEACAM1 is present on the cell surface and can be cleaved or secreted, ensure sample preparation preserves membrane integrity if surface expression is the intended target.
• Since this antibody recognizes an epitope within amino acids 35-428, it may detect multiple isoforms; confirm specificity in your assay system using knockout or knockdown approaches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for targets like CD66a/CEACAM1. For your immunology or oncology assay development, we offer bulk ancillary reagents and can source rare targets upon request. Count on CamelBio as your single procurement partner from concept to clinic.