APC Rabbit anti-Human CD48 Monoclonal Antibody, Glycerol-Free for Flow Cytometry - P09326

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48 (also known as BLAST-1, SLAMF2, BCM1) is a GPI-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with CD244/2B4 and CD2 to regulate immune cell activation and function. Related references: PMID:12007789 PMID:19494291 PMID:27249817
• In T-cells, CD48 associates with CD2 and recruits the Src family kinase LCK and adaptor LAT to the TCR/CD3 complex, promoting LCK phosphorylation and downstream signaling.
• Engagement of CD48 induces phosphorylation of the ITSMs in CD244, triggering formation of the immunological synapse and directed release of cytolytic granules (perforin, granzymes) by T-lymphocytes and NK-cells.
• The protein is primarily localized on the cell membrane and enriched in membrane rafts; a secreted form also exists.
• Widely expressed on all hematopoietic cells, underscoring its role in immune surveillance and intercellular communication.
• Structural features include an immunoglobulin-like domain, disulfide bonds, and a GPI-anchor that mediates membrane attachment. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 27–220), ensuring recognition of native surface CD48; thus, flow cytometry is the primary recommended application.
• This antibody is directly conjugated to APC, allowing immediate use without secondary reagents. However, if indirect detection is desired, select an appropriate anti-rabbit IgG conjugate.
• For best results, validate the antibody in the relevant human hematopoietic sample type (e.g., PBMCs, lymphoid cell lines) and include proper isotype controls and viability staining.
• Minimize cell manipulation and avoid harsh treatments that may disrupt GPI-anchored protein integrity or membrane rafts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. For CD48-focused immune cell research and diagnostic development, our supply chain can support your needs from early-stage R&D to final product integration.