APC Rabbit anti-Human CD47 Monoclonal Antibody for FC - Q08722

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• CD47 (Leukocyte surface antigen CD47; aliases IAP, OA3, MER6) is a 35 kDa transmembrane glycoprotein encoded by the CD47 gene on human chromosome 3, with multiple isoforms generated by alternative splicing.
• It functions as an adhesive protein mediating cell-to-cell interactions and acts as a receptor for thrombospondin-1 (THBS1), modulating integrin signaling through heterotrimeric G proteins. Related references: PMID:11509594, PMID:15383453, PMID:19004835, PMID:7691831, PMID:8550562
• CD47 plays critical roles in apoptosis, angiogenesis, and inflammatory responses; it positively regulates FAS-dependent T-cell apoptosis and suppresses angiogenesis, influencing metabolic regulation during aging. Related references: PMID:32679764
• Interaction with SIRPA on macrophages delivers a "don't eat me" signal, preventing phagocytosis; binding to SIRPG mediates cell-cell adhesion and costimulates T-cell activation. Related references: PMID:11509594, PMID:15383453
• Subcellularly, CD47 is localized to the cell membrane, where it participates in signal transduction, cardiovascular homeostasis, and nitric oxide signaling, contributing to blood pressure regulation (by similarity).
• It is broadly expressed in normal adult tissues, with high levels in epithelia, brain, and macrophages, and is found on ovarian tumors. Related references: PMID:39121194
• CD47 contains an extracellular immunoglobulin-like domain and undergoes N-glycosylation, phosphorylation, and disulfide bond formation, which are essential for its stability and function.

Experimental Guidance and Technical Tips

• The recombinant immunogen corresponds to the extracellular immunoglobulin-like domain (amino acids 19-139), which is exposed on live cells; this design supports flow cytometry applications with intact cells.
• As an APC-conjugated monoclonal antibody, it is ready for direct flow cytometry without secondary reagents; consider titrating to optimize staining for your specific cell model.
• CD47 is expressed on many cell types; use appropriate isotype controls and include blocking agents (e.g., Fc receptor block) to minimize non-specific binding.
• Alternative splicing may result in multiple protein isoforms (~31–35 kDa); if cross-validating by western blot, confirm specificity under reducing/non-reducing conditions.
• Ship on ice and store protected from light at 2–8°C to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering verified antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. This APC-conjugated anti-CD47 antibody supports flow cytometry-based immune profiling, cancer research, and phagocytosis checkpoint studies. For custom requirements, rare target sourcing, or technical consultation, our team delivers tailored procurement solutions from concept to clinic.