APC Rabbit anti-Human CD44 Monoclonal Antibody for FC - P16070

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 (CD44 antigen), also known as PGP-1, ECMR-III, H-CAM, and Hermes antigen, is a cell-surface glycoprotein encoded by the CD44 gene (LHR, MDU2, MDU3, MIC4) in humans.
• Functions as a major receptor for hyaluronan (HA) and other extracellular matrix components, mediating cell-cell and cell-matrix interactions critical for tissue homeostasis. Related references: PMID:16541107 PMID:19703720 PMID:22726066
• Plays essential roles in lymphocyte homing, T-cell activation, hematopoiesis, and inflammatory responses, linking the extracellular environment to intracellular signaling pathways. Related references: PMID:7528188
• Upon ligand engagement, the cytoplasmic domain assembles signaling complexes involving PKN2, RAC1, RHOA, and phospholipase C, promoting calcium mobilization and actin cytoskeleton reorganization for cell migration. Related references: PMID:18757307 PMID:23589287 PMID:15123640
• Facilitates microbial entry: promotes internalization of foot-and-mouth disease virus via macropinocytosis.
• Localizes primarily to the cell membrane and microvilli; also detected as a secreted form. Detected in fibroblasts, urine, and placenta at the protein level. Related references: PMID:25326458 PMID:36213313 PMID:37453717 PMID:32337544
• Undergoes extensive alternative splicing, producing multiple isoforms; heavily modified by glycosylation, phosphorylation, and proteoglycan addition; classified as a blood group antigen and a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–178 within the extracellular domain of human CD44, suggesting recognition of an extracellular epitope; verify target specificity in the relevant cell system.
• This antibody is APC-conjugated and validated for flow cytometry (FC); consider titrating the antibody to determine the optimal working concentration and include appropriate isotype controls.
• Use viable cells and avoid light exposure to the APC conjugate; select appropriate laser/filter settings (excitation 633–640 nm, emission 660 nm).
• For additional applications such as IHC or WB, cross-validation is recommended as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD44-targeted research and diagnostic development, CamelBio provides this APC-conjugated recombinant rabbit monoclonal antibody along with tailored supply solutions for rare targets, supporting your workflow from concept to clinic with consulting and technical services.