APC Rabbit anti-Human CD42b Monoclonal Antibody for FC - P07359

Core Product Specifications and Parameters

Biological Background: CD42b Function and Localization

• GP1BA encodes the platelet glycoprotein Ib alpha chain, also known as CD42b-alpha, a key component of the GPIb-IX-V receptor complex on the platelet surface.
• The protein functions as a receptor for von Willebrand factor (vWF), mediating platelet adhesion to the subendothelium at sites of vascular injury, a critical step in thrombus formation.
• GPIb-alpha is a single-pass type I transmembrane protein, anchored in the platelet membrane, with its extracellular domain containing leucine-rich repeats (LRRs) that bind vWF.
• The protein undergoes post-translational modifications including sulfation, phosphorylation, and disulfide bond formation, which are important for its structure and function.
• Mutations in GP1BA are associated with bleeding disorders such as Bernard-Soulier syndrome and von Willebrand disease, highlighting its essential role in hemostasis.
• The UniProt record classifies it under keywords: Blood coagulation, Cell adhesion, Hemostasis, Membrane, and Disease variant, among others.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 15–531, covering the entire extracellular domain, making the antibody suitable for detecting surface CD42b in flow cytometry without permeabilization.
• For FC applications, titrate the antibody to determine the optimal concentration for your specific cell type and instrument; include appropriate isotype controls and consider live-cell staining protocols to preserve surface epitopes.
• As an APC conjugate, protect the antibody from prolonged light exposure to maintain fluorophore stability; use standard APC filter sets (excitation 633–635 nm, emission 660 nm).
• Validate the antibody in your sample system with a known positive control (e.g., human platelets) and compare with an unstimulated sample to assess expression shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, including this APC-conjugated anti-CD42b antibody for flow cytometry-based platelet research. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support your development pipeline. Leverage our one-stop services to bridge the gap from concept to clinic in hemostasis and thrombosis diagnostics.