APC Rabbit anti-Human CD40 Monoclonal Antibody for FC - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• Official full name: Tumor necrosis factor receptor superfamily member 5 (CD40); also known as B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40.
• Functions as the receptor for TNFSF5/CD40LG, transducing TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to immunoglobulin secretion (By similarity). Related references: PMID:31331973
• Member of the tumor necrosis factor receptor superfamily, characterized by cysteine-rich extracellular repeats and a transmembrane domain.
• Subcellular localization: predominantly a cell membrane single-pass type I membrane protein; a secreted isoform exists due to alternative splicing.
• Tissue specificity: highly expressed on B lymphocytes and detected in primary carcinomas, with broader immune cell expression under activation conditions.
• Keywords and post-translational features: disulfide bond, glycoprotein, alternative splicing, disease variant, signal peptide, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 21-193), suggesting strong recognition of native surface CD40; consider optimal performance in flow cytometry without fixation for live-cell staining.
• For FC, validate the antibody at a range of dilutions (e.g., 0.5–5 µg per 10^6 cells) using positive controls (e.g., human B lymphoblastoid cell lines) and negative controls (e.g., CD40-negative cells).
• If assessing intracellular CD40, include a permeabilization step post-surface staining; note that the immunogen covers the ectodomain but may still detect intracellular isoforms.
• While primarily tested in FC, this monoclonal antibody may cross-perform in other immunoassays (e.g., ELISA, immunohistochemistry) after appropriate optimization; confirm with relevant samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This APC-conjugated anti-human CD40 antibody supports immuno-oncology, autoimmune disease, and B-cell malignancy research. Beyond this reagent, we can supply validated antibody pairs, optimized monoclonal/polyclonal panels, bulk ancillary materials, and rare target sourcing to streamline your assay development and scale-up needs.