APC Rabbit anti-Human CD38 Monoclonal Antibody for FC - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein that serves as an ectoenzyme synthesizing cyclic ADP-ribose (cADPR) from NAD+, a second messenger critical for glucose-induced insulin secretion. Related references: PMID:7961800 PMID:8253715

• It also produces nicotinate-adenine dinucleotide phosphate (NAADP+), a potent Ca2+ mobilizing second messenger, from NADP+ and nicotinic acid. Related references: PMID:16690024

• Beyond enzymatic activity, CD38 functions as a receptor for CD31 on endothelial cells, triggering lymphocyte activation, proliferation, and transendothelial migration. Related references: PMID:9551996 PMID:11259373

• In mature dendritic cells, CD38 engagement modulates CCL21-driven migration, survival, and T helper 1 (Th1)-polarizing activity. Related references: PMID:16293598

• CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, and in malignant lymphomas and neuroblastomas, correlating with its roles in metabolic and immune regulation.

• The protein exists in two alternatively spliced isoforms; the full-length form has a predicted molecular weight of approximately 34 kDa, with glycosylation contributing to observed higher molecular mass.

• Keywords: 3D-structure, alternative splicing, cell membrane, diabetes mellitus, disulfide bond, glycoprotein, hydrolase, NAD, NADP, receptor, signal-anchor, transferase, transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 43-300 of human CD38, which encompasses the extracellular domain, enabling recognition of the native receptor structure on live cells in flow cytometry.
• As a rabbit monoclonal IgG conjugated to APC, this antibody is suitable for single-cell suspension staining; optimal dilution should be determined by titration in the relevant sample system.
• For multicolor flow cytometry panels, ensure appropriate compensation using single-stained controls and consider using a viability dye to exclude dead cells.
• This product has not been validated for applications beyond flow cytometry; testing in other assays would require further experimental optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single access point for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. This APC-labeled anti-CD38 monoclonal antibody supports investigations into calcium signaling, lymphocyte biology, and immune-oncology diagnostics. Our team streamlines procurement to help accelerate your project from concept to clinic.