APC Rabbit anti-Human CD33 Monoclonal Antibody for Flow Cytometry - P20138

Core Product Specifications and Parameters

Biological Background: CD33 (Siglec-3) Function and Localization

• CD33 (Myeloid cell surface antigen CD33) is a transmembrane receptor encoded by the CD33 gene (Gene ID: 945). It is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family, functioning as a cell adhesion and immune regulatory molecule.
• The protein preferentially binds alpha-2,3- and alpha-2,6-linked sialic acid-bearing glycans, mediating cell-cell interactions and maintaining immune cells in a resting state. Related references: PMID:10611343 PMID:11320212 PMID:15597323 PMID:7718872
• Upon ligand engagement, the two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail are phosphorylated by Src-like kinases such as LCK, creating docking sites for phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, which dephosphorylate downstream signaling molecules to attenuate activation. Related references: PMID:10887109 PMID:28325905 PMID:10206955 PMID:10556798
• CD33-mediated inhibition can depend on phosphoinositide 3-kinase (PI3K) activity, contributing to the repression of monocyte activation. Related references: PMID:15597323
• Subcellularly, CD33 localizes to the cell membrane, and it is also detected in peroxisomes, as indicated by its UniProt annotation.
• Tissue expression is restricted to the monocytic/myeloid lineage; in the brain, it is predominantly found on microglial cells.
• Alternative splicing produces multiple isoforms, and the protein is subject to post-translational modifications including glycosylation and phosphorylation. These features are critical for its function and are summarized under keywords such as Glycoprotein, Phosphoprotein, and Disulfide bond.

Experimental Guidance and Technical Tips

• This APC-conjugated rabbit monoclonal antibody was generated using a recombinant immunogen comprising amino acids 18–259 of human CD33, covering the extracellular domain. This design likely targets native epitopes accessible for flow cytometry.
• For FC applications, titrate the antibody to optimize signal-to-noise in your specific cell system; typical starting dilutions require empirical determination.
• CD33 is highly expressed on monocytes and acute myeloid leukemia (AML) blasts, making it a useful marker for immunophenotyping. Validate positively in PBMCs or relevant myeloid cell lines.
• Consider the APC conjugate’s brightness and spectral properties when building multicolor panels, and use appropriate fluorescence compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted B2B partner, CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This APC Rabbit anti-Human CD33 mAb exemplifies our commitment to providing high-quality tools for myeloid cell research and diagnostic development. Whether you need bulk quantities, custom conjugations, or assistance with rare-target sourcing, CamelBio is your dedicated bridge from concept to clinic.