APC Rabbit anti-Human CD275/B7-H2/ICOSL Monoclonal Antibody for FC - O75144

Core Product Specifications and Parameters

Biological Background: ICOS Ligand Function and Localization

• ICOSLG encodes ICOS ligand (also known as B7-H2, B7RP-1, CD275, GL50), a member of the B7 family of immune regulatory proteins. Alternative splicing generates multiple isoforms.
• Functions as a ligand for the T-cell-specific receptor ICOS, delivering costimulatory signals that promote T-cell proliferation and cytokine secretion. Related references: PMID:11007762 PMID:11023515 PMID:30498080
• Induces B-cell proliferation and differentiation into plasma cells, contributing to humoral immunity.
• In endothelial cells, ICOS ligand is essential for proper neutrophil transmigration in response to chemoattractants like CXCL8/IL-8 and fMLP. Related references: PMID:30498080
• Subcellular localization: Cell membrane, consistent with its role as a transmembrane receptor ligand.
• Post-translational modifications: Glycoprotein with disulfide bonds, characteristic of immunoglobulin domain-containing proteins.
• Tissue distribution: Widely expressed at the mRNA level; at the protein level, detected on peripheral blood B-cells, monocytes, monocyte-derived dendritic cells, and within lymph nodes, leukocytes, and spleen.
• Keywords: Adaptive immunity, B-cell activation, Immunoglobulin domain, Transmembrane helix, among others.

Experimental Guidance and Technical Tips

• For flow cytometry, use this APC-conjugated antibody for direct immunofluorescence staining of live human cells to preserve surface ICOS ligand conformation.
• Validate the antibody’s reactivity in your specific experimental system; expression levels can vary by cell type and activation status.
• Because the immunogen spans the extracellular domain (aa 19-256), the antibody likely recognizes the native conformation of surface-expressed CD275.
• Perform optimal antibody titration (e.g., 0.1–5 µg/mL) to achieve the best signal-to-noise ratio and include appropriate isotype controls.
• Avoid fixation or permeabilization steps that may denature the extracellular epitope; if fixation is required, test compatibility beforehand.
• For qualitative analysis, consider a two-step indirect staining protocol if direct conjugation results in insufficient signal.
• Cross-reactivity with non-human species has not been reported; use only for human samples based on current validation data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials and technical support, covering every stage from concept to clinic. This APC-conjugated anti-CD275 monoclonal antibody supports research into immune checkpoint biology and T-cell/B-cell interactions. Through its integrated sourcing platform, CamelBio offers validated antibody pairs, customized monoclonal/polyclonal antibody development, bulk ancillary reagents, and assistance in procuring rare-target raw materials to accelerate your assay development and manufacturing workflows.