APC Rabbit anti-Human CD271/NGFR Monoclonal Antibody for FC - P08138

Core Product Specifications and Parameters

Biological Background: CD271/NGFR Function and Localization

• CD271 (p75NTR/TNFRSF16) is a transmembrane receptor that binds nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NTF3), and neurotrophin-4 (NTF4) with low affinity.
• It forms a high-affinity heterodimeric complex with SORCS2, which preferentially interacts with pro-neurotrophins (proNGF, proBDNF, proNTF3) over mature neurotrophins, playing a critical role in neuronal development and survival. Related references: PMID:24908487
• CD271 mediates both cell survival and apoptosis in a context-dependent manner, thereby regulating specific neuronal populations during development.
• It negatively regulates NF-kappa-B and JNK signaling pathways through interaction with BFAR, and inactivates RHOA, linking to cytoskeletal dynamics and glucose metabolism. Related references: PMID:22566094 PMID:26646181
• CD271 is required for the circadian oscillation of clock genes (BMAL1, PER1, PER2, NR1D1) in the suprachiasmatic nucleus and liver, influencing metabolic gene expression. Related references: PMID:23785138
• In adipocytes and skeletal muscle, CD271 contributes to insulin-stimulated GLUT4 translocation via regulation of RAB31.
• The protein is localized to the cell membrane, cytoplasm, and neuronal projections such as growth cones and dendritic spines.
• Post-translational modifications include glycosylation and phosphorylation; alternative splicing generates multiple isoforms. The observed molecular weight ranges from 35 to 45 kDa depending on glycosylation state.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 29-250), enabling detection of cell surface CD271 by flow cytometry without permeabilization.
• As a directly conjugated APC antibody, no secondary reagent is necessary, reducing background and protocol steps.
• Titration of the antibody is recommended to determine the optimal concentration for your specific sample type and staining conditions.
• Incorporate appropriate isotype controls and Fc receptor blocking to ensure staining specificity.
• For cell surface staining, use live unfixed cells or lightly fixed cells; avoid over-fixation that may mask epitopes.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized supplier of IVD raw materials, CamelBio offers researchers and manufacturers seamless access to validated antibodies like this anti-CD271/NGFR monoclonal antibody, along with a comprehensive portfolio of ancillary reagents, antibody pairs, and rare-target raw materials. Our integrated sourcing services support every stage from concept to clinic, ensuring reliable supply for neurobiology and apoptosis research applications.