APC Rabbit anti-Human CD261/TRAIL-R1 Monoclonal Antibody for FC - O00220

Core Product Specifications and Parameters

Biological Background: CD261/TRAIL-R1 Function and Localization

• TNFRSF10A (CD261, DR4, TRAIL-R1) is a death receptor belonging to the tumor necrosis factor receptor superfamily.
• Acts as receptor for the cytotoxic ligand TNFSF10/TRAIL. Upon ligand binding, recruits FADD and caspase-8 to form the death-inducing signaling complex (DISC), which triggers the apoptotic caspase cascade. Related references: PMID:26457518 PMID:38532423 PMID:19090789
• Promotes activation of NF-kappa-B signaling pathways. Related references: PMID:9430227
• Localizes to the cell membrane, membrane rafts, and cytosol.
• Widely expressed across tissues, with high levels in spleen, peripheral blood leukocytes, small intestine, thymus, and certain cancer cell lines such as K-562 and MCF-7.
• Post-translational modifications include glycosylation, disulfide bond formation, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 124-232) of human CD261/TRAIL-R1, which likely includes the ligand-binding region; consider this when assessing epitope accessibility in native conformations.
• The antibody is directly conjugated to APC, making it suitable for flow cytometry without the need for a secondary detection antibody.
• As CD261/TRAIL-R1 is a cell surface receptor, use gentle cell preparation methods and avoid permeabilization steps that may disrupt membrane localization.
• Include appropriate isotype controls and validate staining in human cell lines or primary cells known to express TRAIL-R1, such as activated T cells or leukemia/breast carcinoma lines.
• Titration experiments are recommended to determine the optimal antibody concentration for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive suite of IVD raw materials and technical support, acting as a single sourcing partner from concept to clinic. For apoptosis and death receptor research, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing. Whether you need conjugates like this APC-labeled antibody or other assay components, CamelBio is ready to streamline your procurement process.