APC Rabbit anti-Human CD244/2B4 Monoclonal Antibody for Flow Cytometry - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244/2B4 Function and Localization

• CD244/2B4 is a heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family and binds the ligand CD48.
• Acts as an activating natural killer (NK) cell receptor, stimulating cytotoxicity, IFN-gamma production, and granule exocytosis. Related references: PMID:10359122 PMID:11714776 PMID:8376943
• Activating function is mediated through association with SH2D1A/SAP and FYN, leading to downstream signaling predominantly via VAV1; signal attenuation in the absence of SH2D1A involves INPP5D. Related references: PMID:15713798 PMID:10934222
• Acts as a costimulator in NK activation by enhancing signals from other NK receptors such as NCR3 and NCR1. Related references: PMID:10741393
• At early stages of NK cell differentiation, may function as an inhibitory receptor to ensure self-tolerance of developing NK cells. Related references: PMID:11917118
• Expressed in all NK cells, monocytes, basophils, TCR-gamma/delta+ T-cells, and on a subset of CD8+ T-cells; tissue expression includes spleen, peripheral blood lymphocytes, lung, liver, testis, and small intestine.
• Localizes to the cell membrane and membrane rafts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–221) of human CD244/2B4; the antibody is expected to recognize the native conformation of the receptor on live cells.
• This APC-conjugated monoclonal antibody is suited for flow cytometry; consider titrating the antibody on human peripheral blood mononuclear cells (PBMCs) or NK cell lines to determine the optimal staining concentration.
• For multicolor flow cytometry panels, verify compensation settings and use appropriate isotype controls to assess background staining.
• Fc receptor blocking may be advisable to reduce non-specific binding, especially in monocyte and basophil populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and optimized monoclonal/polyclonal antibodies for immune cell targets. Our CD244/2B4 antibody offerings are suited for flow cytometric analysis of NK and T-cell function in immunology research and clinical assay development. Leverage our streamlined sourcing for rare target reagents, bulk ancillaries, and custom procurement—backed by technical consultation to accelerate your project from concept to clinical application.