APC Rabbit anti-Human CD213a1/IL-13Rα1 Monoclonal Antibody for FC - P78552

Core Product Specifications and Parameters

Biological Background: IL-13Rα1 Function and Localization

• Interleukin-13 receptor subunit alpha-1 (IL-13Rα1), also known as cancer/testis antigen 19 (CT19), is a cytokine receptor encoded by the IL13RA1 gene. Alternative splicing may generate multiple isoforms.
• IL-13Rα1 binds interleukin-13 (IL-13) with low affinity and forms a functional receptor complex with IL4RA, enabling high-affinity IL-13 binding and signaling.
• The protein also acts as an alternate accessory protein for interleukin-4 (IL-4) signaling, interacting with IL4RA, but cannot substitute for IL2RG in mediating enhanced interleukin-2 (IL-2) binding.
• IL-13Rα1 is a transmembrane glycoprotein localized at the cell membrane, featuring disulfide bonds and glycosylation that are important for its structure and function.
• Ubiquitously expressed, IL-13Rα1 shows highest levels in heart, liver, skeletal muscle, and ovary, with lower expression in brain, lung, and kidney; it is also found in B-cells, T-cells, and endothelial cells.
• As a cancer/testis antigen, IL-13Rα1 is aberrantly expressed in various tumors, making it a potential target for cancer immunotherapy and diagnostic applications.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 29-343), suggesting the antibody recognizes an extracellular epitope, which is ideal for flow cytometry on live, non-permeabilized cells.
• For flow cytometry, use an appropriate isotype control and optimize cell staining conditions (e.g., antibody concentration, incubation time, temperature) using a known positive human cell line.
• Because the antibody is pre-conjugated with APC, it enables direct detection without secondary antibodies, reducing background and simplifying the workflow.
• Validate the antibody in the relevant sample system, considering the ubiquitous but variable expression of IL-13Rα1; human PBMCs, cultured endothelial cells, or tumor cell lines may serve as suitable positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and expert consulting. For projects targeting IL-13Rα1 and related cytokine receptor pathways, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—streamlining your journey from concept to clinic.