APC Rabbit anti-Human CD209/DC-SIGN Monoclonal Antibody for FC - Q9NNX6

Core Product Specifications and Parameters

Biological Background: CD209/DC-SIGN Function and Localization

• CD209, also known as DC-SIGN or CLEC4L, is a C-type lectin receptor characterized by a single carbohydrate recognition domain and alternative splicing variants.
• It functions as a pathogen-recognition receptor on the surface of immature dendritic cells, mediating endocytosis of pathogens for lysosomal degradation and subsequent antigen presentation via MHC class II to T-cells.
• Through calcium-dependent mannose-binding, CD209 serves as a high-affinity receptor for ICAM2 and ICAM3, enabling dendritic cell transendothelial migration and facilitating primary DC‑T cell clustering and T‑cell proliferation. Related references: PMID:10721994, PMID:10721995, PMID:11017109
• Acts as an attachment receptor for a broad range of viruses, including HIV‑1/2, Ebolavirus, Cytomegalovirus, HCV, Dengue virus, Measles virus, HSV‑1, Influenza A, SARS‑CoV, Japanese encephalitis virus, Lassa virus, Marburg virus, RSV, Rift Valley fever virus, Uukuniemi virus, and West Nile virus, highlighting its role in host–pathogen interactions. Related references: PMID:23966408
• Recognizes mannose‑containing glycoconjugates on bacterial pathogens such as Mycobacterium tuberculosis (ManLAM), Helicobacter pylori (Lewis‑x), Klebsiella pneumoniae, and others, contributing to innate immune recognition of bacteria. Related references: PMID:16379498, PMID:16092920, PMID:21203928
• Subcellular localization is primarily at the cell membrane and membrane rafts; secreted isoforms are also reported, as indicated by UniProt annotations.
• Predominantly expressed in dendritic cells and DC‑residing tissues, placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP‑1 monocytes.
• Post‑translational modifications include N‑linked glycosylation and disulfide bond formation; the protein is annotated with keywords such as “Alternative splicing,” “Glycoprotein,” and “Calcium” in UniProt.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 59 to 404 of human CD209, covering most of the extracellular domain. This design may allow detection of both membrane-bound and certain soluble forms, but users should verify specificity in their specific assay system.
• Recommended for flow cytometry (FC) on live or fixed human dendritic cells or CD209‑expressing cell lines. Because CD209 expression can vary with maturation state, consider titrating the antibody and using appropriate isotype controls.
• For multi‑color panels, the APC conjugate enables easy combination with FITC, PE, or PerCP‑labeled antibodies. Ensure adequate compensation settings.
• Given the molecule’s role as a viral and bacterial receptor, this antibody may also be useful in functional studies (e.g., blocking assays), but such applications have not been validated and require thorough optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, from validated monoclonal antibodies to bulk ancillary reagents. This APC anti‑CD209/DC‑SIGN monoclonal antibody exemplifies our capability to supply well‑characterized tools for dendritic cell biology, immunology, and host‑pathogen interaction studies. Beyond individual antibodies, we offer customized antibody development, matched antibody pairs, and rare‑target sourcing to streamline your diagnostic assay development from concept to clinic.