APC Rabbit anti-Human CD1B Monoclonal Antibody for Flow Cytometry - P29016

Core Product Specifications and Parameters

Biological Background: CD1b Function and Localization

• CD1b is a T-cell surface glycoprotein and member of the CD1 family of antigen-presenting molecules.
• It binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells, playing a central role in adaptive immunity.
• The protein is expressed as a type I membrane protein with a single transmembrane helix, an extracellular domain containing immunoglobulin-like domains, and a short cytoplasmic tail.
• CD1b localizes to the cell membrane as well as to endosome and lysosome membranes, facilitating the presentation of lipid antigens derived from intracellular pathogens.
• It is expressed on cortical thymocytes, certain T-cell leukemias, and in various other tissues, indicating a role in immune surveillance and T-cell development.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for proper folding and antigen binding.
• Alternative splicing generates multiple isoforms, as indicated by proteomics identification and reference proteome data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18-303 of human CD1B, which encompasses the extracellular domain; this design suggests the antibody may recognize native CD1b on the cell surface.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type and staining protocol.
• As this antibody is directly conjugated to APC, ensure your flow cytometer is equipped with a suitable laser (e.g., 633–635 nm) and filter set for APC detection.
• Validate antibody specificity in your system by including appropriate isotype controls and, if possible, using CD1b-deficient cells as negative controls.
• Cross-reactivity has been confirmed for human; reactivity with other species has not been tested. If using in non-human models, verify cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. With our validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we support your CD1b-related research and assay development from concept to clinic. Our supply chain expertise ensures reliable sourcing for rare target raw materials, enabling you to focus on your core innovation.