APC Rabbit anti-Human CD196/CCR6 Monoclonal Antibody for FC - P51684

Core Product Specifications and Parameters

Biological Background: CD196/CCR6 Function and Localization

• CCR6 is a receptor for the C-C chemokine CCL20 (PubMed:9169459), and ligand binding triggers intracellular calcium ion influx (PubMed:20068036).
  Related references: PMID:9169459 PMID:20068036

• It also serves as a receptor for beta-defensins, binding DEFB1 to regulate sperm motility and bactericidal activity (PubMed:25122636), and mediating chemotactic responses via DEFB4/DEFB4A/B (PubMed:20068036).
  Related references: PMID:25122636 PMID:20068036

• The CCL20-CCR6 pair directs chemotaxis of dendritic cells, effector/memory T-cells, and B-cells, playing a critical role in skin and mucosal immune homeostasis and inflammation (PubMed:21376174).
  Related references: PMID:21376174

• CCR6 is essential for recruiting both pro-inflammatory Th17 cells and regulatory T-cells (Tregs) to inflammatory sites, and for normal Th17 cell migration in intestinal Peyer's patches.

• In B-cell biology, CCR6 is required for B-cell localization in the subepithelial dome of Peyer's patches, efficient IgA class switching, proper anatomical distribution of memory B-cells in the spleen, and secondary recall responses.

• In the reproductive system, CCR6 positively regulates sperm motility and chemotaxis via CCL20 binding (PubMed:23765988).
  Related references: PMID:23765988

• Subcellularly, CCR6 is located at the cell membrane and cell surface, consistent with its role as a transmembrane receptor.

• CCR6 is expressed in spleen, lymph nodes, appendix, fetal liver, and in T-cells and B-cells, but not in NK cells, monocytes, or granulocytes. In sperm, it localizes primarily to the tail and postacrosomal region.

• Structurally, CCR6 is a G protein-coupled receptor with seven transmembrane helices, containing disulfide bonds, glycosylation sites, and proteolytic processing features.

Experimental Guidance and Technical Tips

• As an APC-conjugated antibody, it is ready-to-use for direct flow cytometry. Protect from prolonged light exposure to preserve fluorophore stability.
• Optimize staining by titrating the antibody over a range (e.g., 0.1–10 µg/mL) using a relevant cell line or primary cells known to express CCR6, such as T-cell subsets or dendritic cells.
• Include appropriate isotype controls and a viability dye to exclude dead cells, and consider Fc receptor blocking for samples with high Fc receptor expression.
• For intracellular staining of CCR6 (e.g., if analyzing cytokine co-expression), validate fixation and permeabilization conditions; note that APC is compatible with commonly used buffers.
• Validate performance in the specific sample matrix (e.g., whole blood, PBMCs, tissue digests) by assessing signal-to-noise ratio and resolution of positive versus negative populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CD196/CCR6 antibody addresses the growing need for reliable chemokine receptor detection in immunological assays. As your sourcing partner, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare-target raw-material procurement to accelerate your diagnostic and research workflows.