APC Rabbit anti-Human CD185/CXCR5 Monoclonal Antibody for FC - P32302

Core Product Specifications and Parameters

Biological Background: CXCR5 Function and Localization

• CXCR5 (C-X-C chemokine receptor type 5), also designated CD185, BLR1, and MDR15, is a seven-transmembrane G protein-coupled receptor (GPCR) encoded by the CXCR5 gene.
• It functions as a specific receptor for B-lymphocyte chemoattractant (BLC/CXCL13), driving B-cell migration into the B-cell follicles of spleen and Peyer's patches.
• CXCR5 is implicated in the regulation of B-cell differentiation and may play a role in Burkitt lymphoma lymphomagenesis.
• The receptor is localized on the cell membrane.
• Expression is predominantly observed in mature B-cells and Burkitt lymphoma cells.
• Structural features include an extracellular N-terminus, disulfide bonds, and glycosylation, typical of chemokine receptors.
• Alternative splicing generates multiple isoforms of CXCR5.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1–47), making it suitable for detecting surface-expressed CXCR5 by flow cytometry (FC).
• For FC experiments, consider using an appropriate isotype control to assess non-specific binding.
• Validate the antibody’s performance in your specific sample system, such as human peripheral blood B-cells or Burkitt lymphoma cell lines.
• Titrate the antibody to determine the optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research labs with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This APC-conjugated anti-human CXCR5 monoclonal antibody exemplifies our offering for immunology and oncology research applications. We also specialize in sourcing rare-target materials to support your entire development pipeline, from concept to clinical use.