APC Rabbit anti-Human CD172a/SIRPα Monoclonal Antibody for Flow Cytometry - P78324

Core Product Specifications and Parameters

Biological Background: SIRPα/CD172a Function and Localization

• Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPα, also known as SHPS-1, CD172a, BIT, MYD-1, p84) is an immunoglobulin-like cell surface receptor that acts as a docking protein for PTPN6 and PTPN11, inducing their translocation from cytosol to plasma membrane.
• Functions as a receptor for CD47, mediating negative regulation of phagocytosis, mast cell activation, and dendritic cell activation, and inhibiting maturation and cytokine production by dendritic cells upon CD47 binding.
• Supports cerebellar neuron adhesion, neurite outgrowth, and glial cell attachment, suggesting roles in synaptogenesis and synaptic function.
• Acts as a receptor for thrombospondin-1 (THBS1), which stimulates SIRPA phosphorylation and triggers NADPH oxidase-derived ROS production in non-phagocytic cells. Related references: PMID:24511121
• Exists as three main isoforms (Sirp-alpha-1, -2, -3) due to alternative splicing, with ubiquitous tissue expression but highest levels in brain and on myeloid cells, and absent on T-cells.
• Localizes to the cell membrane as a single-pass type I membrane protein, with an extracellular region containing three immunoglobulin-like domains and a cytoplasmic tail with tyrosine phosphorylation sites.
• Heavily glycosylated and phosphorylated, playing a role in antiviral immunity by limiting new world arenavirus internalization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 31–370, which comprises the entire extracellular domain; therefore, the antibody is expected to recognize the native conformation of SIRPα on the cell surface.
• As an APC-conjugated monoclonal antibody, it is ready for direct immunofluorescence staining in flow cytometry without the need for a secondary detection reagent.
• For optimal staining, titrate the antibody on the target cell type (e.g., human monocytes, macrophages, or dendritic cell lines) to determine the working concentration.
• Include an appropriate isotype control (APC-conjugated rabbit IgG matched clone) and, if using whole blood or tissue-derived cell suspensions, pre-incubate with Fc receptor blocking reagent to minimize non-specific binding.
• Since SIRPα is not expressed on T-cells, they can serve as an internal negative control; validate specificity under your experimental conditions using known positive and negative cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical support. For studies centered on SIRPα/CD172a in immune regulation, phagocytosis, or tumor microenvironment research, CamelBio can assist in sourcing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target-specific raw materials to accelerate your assay development pipeline.