APC Rabbit anti-Human CD162/PSGL-1 Monoclonal Antibody for Flow Cytometry - Q14242

Core Product Specifications and Parameters

Biological Background: P-selectin glycoprotein ligand 1 (CD162/PSGL-1) Function and Localization

• CD162/PSGL-1 is a SLe(x)-type proteoglycan that serves as the primary ligand for P-, E-, and L-selectins, mediating rapid leukocyte rolling on vascular endothelium during inflammation.
• The protein exists as multiple isoforms due to alternative splicing, which may modulate its binding properties or expression.
• It is predominantly expressed on the surface of neutrophils, monocytes, and most lymphocytes, playing a critical role in leukocyte trafficking.
• Subcellular localization is primarily at the plasma membrane (Membrane; Single-pass type I membrane protein).
• PSGL-1 is heavily post-translationally modified, including sulfation, sialylation, and O-glycosylation, which are essential for selectin binding; additional modifications include phosphorylation, disulfide bond formation, and N-terminal pyroglutamic acid formation.
• In addition to its role in cell adhesion, PSGL-1 acts as a receptor for enterovirus 71, facilitating viral entry into host cells.
• The protein contains a signal peptide, an extracellular mucin-like domain with decameric repeats, a transmembrane helix, and a cytoplasmic tail.

Experimental Guidance and Technical Tips

• As the immunogen corresponds to the extracellular domain (aa 42-295), the resulting antibody is expected to recognize the native transmembrane form of CD162/PSGL-1 on live cells, making it particularly suitable for flow cytometry (FC).
• For FC applications, optimize antibody titration and consider using appropriate isotype controls to ensure specificity, especially given the potential for Fc receptor binding on leukocytes.
• Validate expression of CD162 in target cell populations (e.g., peripheral blood leukocytes, activated T cells, or appropriate cell lines) prior to use, as expression levels can vary with cell activation state.
• Since PSGL-1 is a heavily glycosylated protein, the epitope recognized may be conformation-dependent; denaturing conditions might affect antibody binding in alternative applications like western blotting.
• The APC conjugate enables direct fluorescence detection, eliminating the need for secondary reagents; ensure proper compensation in multicolor flow cytometry panels to avoid spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our CD162/PSGL-1 antibody offerings are part of a broader immunology and inflammation panel, enabling leukocyte adhesion and trafficking studies. Through our integrated sourcing network, we assist diagnostic manufacturers and research labs in accessing rare target reagents and customized solutions from concept to clinic.