APC Rabbit anti-Human CD161 Monoclonal Antibody for FC - Q12918

Core Product Specifications and Parameters

Biological Background: Killer cell lectin-like receptor subfamily B member 1 Function and Localization

• CD161, also known as NKRP1A or KLRB1, is a killer cell lectin-like receptor subfamily B member 1 that acts as an inhibitory receptor on natural killer (NK) cells.
• Activation of CD161 leads to stimulation of acid sphingomyelinase/SMPD1, resulting in elevated intracellular ceramide levels, and also triggers AKT1/PKB and RPS6KA1/RSK1 kinase signaling, enhancing T-cell proliferation induced by anti-CD3.
• CD161 functions as a lectin, binding to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and the N-acetyllactosamine epitope, and interacts with CLEC2D/LLT1 as a ligand to inhibit NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells.
• The receptor localizes to the cell membrane and is characterized as a single-pass type II membrane protein (signal-anchor) with a C-type lectin domain.
• Tissue expression is restricted to a subset of NK cells predominantly found in intestinal epithelium and liver, and it is also present on peripheral blood T-cells, especially those with a memory antigenic phenotype.
• Post-translational modifications include glycosylation, and the protein contains disulfide bonds essential for its structure; the molecular weight of the unmodified protein is approximately 25.4 kDa.
• Keywords from UniProt: 3D-structure, Disulfide bond, Glycoprotein, Lectin, Membrane, Receptor, Signal-anchor, Transmembrane helix.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is recommended for flow cytometry (FC). Optimize the working concentration by titrating on relevant cell types, such as NK cells or memory T-cells, to achieve optimal signal-to-noise ratio.
• The immunogen corresponds to amino acids 67-225, which lies within the extracellular lectin-like domain. Ensure that the epitope is accessible on the cell surface in your experimental setup.
• Since CD161 is expressed on a subset of NK cells and memory T-cells, include appropriate cell lineage markers in multicolor flow cytometry panels to correctly gate the population of interest.
• Validate antibody performance under your specific conditions (e.g., cell activation states, fixation/permeabilization protocols) as these may affect epitope presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This APC Rabbit anti-Human CD161 mAb is an example of our offering for immunophenotyping and NK cell research, supporting the development of flow cytometry-based diagnostics. Our one-stop sourcing model streamlines procurement—from rare-target antibodies to bulk reagent supply—helping you accelerate assay development from concept to clinic.