APC Rabbit anti-Human CD123 Monoclonal Antibody for Flow Cytometry - P26951

Core Product Specifications and Parameters

Biological Background: CD123 Function and Localization

• CD123, also known as interleukin-3 receptor subunit alpha (IL-3Rα), is the ligand-binding alpha chain of the IL-3 receptor, which heterodimerizes with the common beta subunit (IL3RB) upon IL-3 engagement.
• It is primarily expressed on hematopoietic progenitor cells, monocytes, and B-lymphocytes, playing a critical role in hematopoiesis and lineage commitment (PubMed:10527461). Related references: PMID:10527461
• IL-3 binding triggers intracellular signaling via JAK2 and PI3K pathways, ultimately activating STAT5-mediated transcription to regulate cell proliferation and differentiation (By similarity).
• The receptor is localized to the cell membrane and contains an extracellular domain (aa 1-378 used as the immunogen), a single transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitin-like conjugation, influencing receptor stability and signaling.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• The molecular weight is approximately 43 kDa (UniProt: 43330 Da), consistent with the glycosylated form observed in Western blot.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein, aa 1-378) corresponds to the extracellular domain of human CD123, ensuring recognition of the native surface receptor; suitable for flow cytometry (FC) applications.
• For FC, titrate the APC-conjugated antibody using relevant hematopoietic cells (e.g., peripheral blood mononuclear cells, leukemia cell lines) to determine optimal staining concentrations.
• Include appropriate isotype controls and compensate for autofluorescence if analyzing primary samples.
• Although formulated for FC, the antibody may be evaluated in other immunoassays with proper validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic developers. For CD123-related hematopoietic research and diagnostic applications, we offer comprehensive support, from concept to clinic. Our team can also assist in sourcing rare targets and custom antibody development to meet your specific needs.