APC Rabbit anti-Human CD11a Monoclonal Antibody for FC - P20701

Core Product Specifications and Parameters

Biological Background: Integrin alpha-L Function and Localization

• ITGAL encodes integrin alpha-L (CD11a), a leukocyte-specific adhesion receptor that pairs with integrin beta-2 (ITGB2) to form lymphocyte function-associated antigen 1 (LFA-1).
• LFA-1 binds multiple ligands: ICAM1 (PubMed:10846180), ICAM2 (PubMed:1676048), ICAM3 (PubMed:12526797), ICAM4, F11R (PubMed:11812992; PubMed:15528364), and ISG15 (PubMed:29100055), mediating leukocyte adhesion, transmigration, and immune synapse formation (PubMed:38195629). Related references: PMID:10846180 PMID:12526797 PMID:1676048 PMID:11812992 PMID:15528364 PMID:29100055 PMID:38195629
• It contributes to cytotoxic T-cell and NK cell killing (PubMed:15356110), antibody-dependent cellular cytotoxicity, and phagocytosis of apoptotic neutrophils via ICAM3 (PubMed:23775590), and is required for lymphopoiesis (By similarity). Related references: PMID:15356110 PMID:23775590
• Localized to the plasma membrane and membrane rafts, consistent with its role in cell adhesion and signaling.
• The protein is heavily glycosylated, contains disulfide bonds, and undergoes phosphorylation; it binds calcium and magnesium, essential for integrin function.
• Expressed exclusively on leukocytes, where it mediates immune surveillance by facilitating leukocyte-endothelial interactions and transendothelial migration.
• Additionally, LFA-1 interacts with neuron-specific ICAM5 to enable neuron-leukocyte adhesion (PubMed:10741396), highlighting its role in neuroimmune communication. Related references: PMID:10741396

Experimental Guidance and Technical Tips

• Titrate the antibody to determine the optimal concentration for flow cytometry, and include appropriate isotype controls to assess non-specific binding.
• Since CD11a is a cell surface antigen, permeabilization is not required; consider blocking Fc receptors to reduce background in leukocyte samples.
• The reported molecular weight range (118–134 kDa) likely reflects glycosylation heterogeneity; validate the observed band in your specific sample system if performing western blot.
• For multi-color flow panels, verify compensation settings with your instrument configuration, as APC is a bright fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including this APC-conjugated anti-CD11a monoclonal antibody for leukocyte adhesion and immune function studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—streamlining your development from concept to clinic.