APC Rabbit anti-Human CD10 Monoclonal Antibody for Flow Cytometry - P08473

Core Product Specifications and Parameters

Biological Background: CD10 (Neprilysin) Function and Localization

• CD10, also known as neprilysin (NEP), common acute lymphocytic leukemia antigen (CALLA), enkephalinase, and skin fibroblast elastase (SFE), is a zinc-dependent metalloprotease encoded by the MME gene.
• It exhibits thermolysin-like specificity, predominantly cleaving polypeptides of up to 30 amino acids.
  Related references: PMID:15283675, PMID:6208535, PMID:6349683
• Neprilysin inactivates opioid peptides such as Met- and Leu-enkephalins by cleaving the Gly-Phe bond, thereby modulating nociception.
  Related references: PMID:17101991, PMID:6349683
• It catalyzes the degradation of bradykinin, substance P, and neurotensin.
  Related references: PMID:6208535
• The enzyme cleaves angiotensin-1, angiotensin-2, and angiotensin 1-9, influencing the renin-angiotensin system.
  Related references: PMID:15283675, PMID:6349683
• Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP), neprilysin plays a role in cardiovascular homeostasis.
  Related references: PMID:16254193, PMID:2531377, PMID:2972276
• It displays UV-inducible elastase activity toward skin preelastic and elastic fibers, implicating it in skin aging.
  Related references: PMID:20876573
• CD10 is a single-pass type II integral membrane protein localized at the cell surface, with a large extracellular domain for substrate binding.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 52–750) encompasses the majority of CD10's extracellular domain, suggesting that this antibody may recognize the native protein on live cells.
• As a directly conjugated APC rabbit monoclonal antibody, it is primarily designed for flow cytometry; consider titrating for your specific cell system.
• CD10 is a cell surface glycoprotein; for FC assays, confirm cell surface expression and include relevant isotype and unstained controls.
• The antibody specificity is reported for human; cross-reactivity with other species has not been determined.

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