APC Mouse anti-Human TCR α/β Monoclonal Antibody for FC - P01848

Core Product Specifications and Parameters

Biological Background: TCR Alpha Constant Region Function and Localization

• The T cell receptor alpha chain constant region (TRAC), encoded by the TRAC gene, is a core component of the αβ TCR, which recognizes peptide-MHC complexes on antigen-presenting cells to initiate adaptive immune responses (PubMed:24600447, 25493333). Related references: PMID:24600447 and PMID:25493333.
• Antigen engagement triggers CD3 complex clustering and downstream signaling via LCK, ZAP70, and the LAT signalosome, activating calcium, MAPK, and NF-κB pathways critical for T cell gene expression and differentiation (PubMed:23524462). Related references: PMID:23524462.
• The TCR repertoire is generated in the thymus by V-(D)-J recombination and shaped by intrathymic selection to ensure self-MHC restriction and prevent autoimmunity (PubMed:15040585). Related references: PMID:15040585.
• Post-thymic interactions with pMHC complexes further modulate TCR structural and functional avidity, fine-tuning T cell responsiveness.
• TRAC is a transmembrane glycoprotein located on the cell surface, featuring a conserved extracellular immunoglobulin-like domain stabilized by a disulfide bond.
• It participates in the multimeric TCR-CD3 receptor complex and is classified under adaptive immunity, T cell receptor, and glycoprotein keywords.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering human TCR α/β; the antibody likely targets the constant region, enabling broad detection of αβ T cells independent of variable gene usage.
• As an APC-conjugated antibody, it is suitable for direct flow cytometry staining; consider titrating the antibody on your target cell population to optimize resolution.
• Include appropriate isotype controls and viability dyes in your panel to ensure specific staining and accurate gating of live TCR α/β+ cells.
• Cross-reactivity has been confirmed with human; performance in other species should be validated if required for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with end-to-end access to IVD raw materials, from validated antibody pairs and bulk ancillary reagents to customized sourcing of rare targets. This APC-conjugated anti-human TCR α/β antibody fits into T cell immunophenotyping and immune monitoring workflows. Our supply capabilities help streamline your assay development with reliable raw materials and technical consultation.