APC Mouse anti-Human CD62P/P-selectin mAb - P16109

Core Product Specifications and Parameters

Biological Background: P-selectin Function and Localization

• P-selectin (CD62P, also known as GMP-140, LECAM3, PADGEM) is a single-pass type I membrane protein encoded by the SELP gene. It belongs to the selectin family of adhesion molecules.
• Functions as a Ca²⁺-dependent receptor that binds to carbohydrate ligands (sialyl-Lewis X) on neutrophils and monocytes, facilitating leukocyte rolling and the initial steps of inflammation.
• Mediates the interaction between activated endothelial cells or platelets and leukocytes through its counter-receptor SELPLG (P-selectin glycoprotein ligand-1).
• Also interacts with integrins alpha-IIb/beta3 (ITGA2B:ITGB3) and alpha-V/beta-3 (ITGAV:ITGB3) to promote stable cell adhesion. Related references: PMID:37184585
• Subcellular location: Predominantly stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cellular activation by agonists, it is rapidly translocated to the cell membrane.
• Contains structural features including a lectin domain, an EGF-like domain, multiple sushi (SCR) repeats, and a transmembrane helix. Post-translational modifications include disulfide bond formation, glycosylation, and palmitoylation.

Experimental Guidance and Technical Tips

• The APC conjugate allows direct flow cytometry detection without secondary antibody steps, minimizing background and saving time. Validate the staining in the relevant sample system (e.g., activated platelets or endothelial cells).
• For optimal results, consider using a positive control such as thrombin-activated human platelets or histamine-stimulated endothelial cells, which upregulate surface P-selectin.
• The monoclonal nature of this antibody provides high specificity for the extracellular domain of human CD62P. As with any immunological reagent, titrate the antibody to determine the optimal working concentration for your specific flow cytometry protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering one-stop access to validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This APC-conjugated anti-human CD62P/P-selectin antibody supports research into inflammation, thrombosis, and cell adhesion, and is just one example of how we facilitate your assay development from concept to clinic. Whether you need monoclonal antibodies, recombinant proteins, or technical consulting, CamelBio streamlines your supply chain for diagnostic manufacturing and life science research.