APC Mouse anti-Human CD56 Monoclonal Antibody for FC - P13591

Core Product Specifications and Parameters

Biological Background: CD56 (NCAM1) Function and Localization

• Neural cell adhesion molecule 1 (NCAM1), also known as CD56, is a member of the immunoglobulin superfamily mediating both homophilic and heterophilic cell–cell adhesion.
• It plays essential roles in neural development, including neurite outgrowth, fasciculation, and synaptic plasticity, contributing to learning and memory.
• NCAM1 undergoes extensive alternative splicing, producing multiple protein isoforms with molecular weights ranging from ~40 kDa to ~95 kDa observed in human tissues.
• The protein is primarily localized at the cell membrane, but secreted isoforms lacking the transmembrane domain also exist and contribute to extracellular matrix interactions.
• Post-translational modifications include glycosylation (polysialic acid attachment) and phosphorylation, which modulate adhesive properties and intracellular signaling.
• NCAM1 serves as a host cell receptor for rabies virus and Zika virus, facilitating viral entry during infection.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is designed for flow cytometry; verify optimal dilution and staining conditions on representative human cell lines or primary cells.
• Consider including appropriate isotype controls and viability dyes to ensure specific detection of CD56 on the cell surface.
• Given the presence of multiple isoforms, confirm that the antibody recognizes the expected target in your specific experimental system via additional characterization (e.g., Western blot on cell lysates).
• For detection of secreted or shed forms, standard flow cytometry protocols may require adaptation; alternative assays may be more suitable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—every step from concept to clinic. For studies involving neural adhesion, viral pathogenesis, or NK cell biology, we supply high-quality antibodies such as this APC-conjugated anti-CD56 monoclonal antibody, alongside validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. Leverage our integrated supply chain to accelerate your diagnostic and research applications.