APC Mouse Anti-Human CD42a (GPIX) Monoclonal Antibody for Flow Cytometry - P14770

Core Product Specifications and Parameters

Biological Background: CD42a (GPIX) Function and Localization

• CD42a, also known as platelet glycoprotein IX (GPIX) or glycoprotein 9, is a membrane protein encoded by the GP9 gene in humans.
• GPIX is a subunit of the GPIb-V-IX complex, which serves as a receptor for von Willebrand factor (vWF) and mediates vWF-dependent platelet adhesion to blood vessels.
• This adhesion is a critical initiating event in hemostasis, particularly at sites of vascular injury in the arterial circulation.
• GPIX contains a leucine-rich repeat (LRR) domain, a signal peptide, a transmembrane helix, and a disulfide bond, contributing to its structural role in the complex.
• Subcellularly, GPIX localizes to the plasma membrane of platelets, where it may provide membrane insertion and proper orientation of GPIb within the complex.
• Mutations in GP9 are associated with Bernard-Soulier syndrome, a rare inherited bleeding disorder characterized by abnormal platelet adhesion and prolonged bleeding time.
• Keywords from UniProt include: blood coagulation, cell adhesion, glycoprotein, hemostasis, leucine-rich repeat, membrane, transmembrane, and disease variant.

Experimental Guidance and Technical Tips

• Given the immunogen is recombinant human CD42a protein, this antibody is expected to specifically recognize human CD42a (GPIX) in native and denatured conditions. Confirm specificity by testing on appropriate positive and negative cell lines or platelet samples.
• For flow cytometry (FC), titrate the antibody to determine the optimal working concentration. Use appropriate isotype controls and consider employing platelet-rich plasma or washed platelets as sample material, gating on platelet population based on forward/side scatter and additional platelet markers.
• The conjugate APC is suitable for multi-color flow cytometry panels; verify compensation settings and avoid freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a comprehensive one-stop sourcing partner for IVD raw materials, providing researchers and diagnostic manufacturers with reliable access to antibodies, antigens, and ancillary reagents for hemostasis and platelet biology research. From this CD42a (GPIX) monoclonal antibody to optimized antibody pairs and bulk buffers, our supply chain supports every stage of assay development and production. Whether you need rare target reagents or large-scale custom sourcing, CamelBio bridges the gap from concept to clinic.