APC Mouse anti-Human CD3 Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 epsilon Function and Localization

• CD3E (CD3 epsilon chain, aliases T3E, TCRE, IMD18) is a subunit of the TCR-CD3 complex expressed on T cells, crucial for adaptive immunity. Related references: PMID:15294938 PMID:15546002 PMID:2470098

• Upon TCR engagement, CD3E, along with other CD3 chains, transduces activation signals across the membrane via its immunoreceptor tyrosine-based activation motifs (ITAMs). Related references: PMID:2470098 PMID:40592325

• ITAM phosphorylation by LCK and FYN creates docking sites for ZAP70, triggering downstream signaling cascades including calcium flux and MAPK/ERK pathways. Related references: PMID:2470098 PMID:40592325

• CD3E is essential for T-cell development; it participates in thymocyte selection and maturation.

• It contains endocytosis motifs that mediate internalization and surface down-regulation of the TCR-CD3 complex, regulating signal duration. Related references: PMID:10384095 PMID:26507128

• CD3E also serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 interaction and blocking ZAP70 binding and NF-kB signaling. Related references: PMID:12110186 PMID:38614099

• Subcellularly, CD3E is localized to the cell membrane, consistent with its role in extracellular ligand recognition and signal transduction.

• It is characterized by a signal peptide, immunoglobulin domain, transmembrane helix, and cytoplasmic ITAM motifs; it undergoes phosphorylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine optimal staining concentration for your cell population; typically 0.5–5 µg per 1×10⁶ cells is a starting point.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity.
• APC conjugate is excitable by 633–647 nm lasers; minimize light exposure to prevent photobleaching.
• As a recombinant protein-derived antibody, it recognizes the native CD3 epsilon chain; fixation/permeabilization may be required for intracellular epitopes.
• This antibody is validated for human samples; cross-reactivity with other species has not been determined, so test in relevant model systems if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-human CD3 antibody suits immune monitoring and T-cell profiling applications. For customized IVD development—from concept to clinic—partner with CamelBio for streamlined, quality-assured material supply.