APC Mouse Anti-Human CD11b Monoclonal Antibody for Flow Cytometry - P11215

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (ITGAM/CD11b) combines with integrin beta-2 (ITGB2/CD18) to form the Mac-1/CR3 heterodimer, mediating adhesive interactions of monocytes, macrophages, and granulocytes.
• Acts as a receptor for the iC3b fragment of complement component C3, facilitating the uptake of complement-coated particles and pathogens. Related references: PMID:20008295, PMID:9558116
• Recognizes ligands including fibrinogen and factor X, possibly via the R-G-D peptide, and binds the P1 and P2 peptides of fibrinogen gamma chain.
• Functions as a receptor for ICAM1 on stimulated endothelial cells, promoting leukocyte adhesion. Related references: PMID:1980124
• Regulates neutrophil migration and is required, in association with ITGB2, for CD177-PRTN3-mediated activation of TNF-primed neutrophils. Related references: PMID:28807980, PMID:21193407
• In microglia, associates with TYROBP/DAP12 to control superoxide ion production, contributing to neuronal apoptosis during brain development (By similarity).
• Undergoes alternative splicing and contains characteristic integrin features, including extracellular calcium-binding repeats, disulfide bonds, and glycosylation sites.
• Predominantly expressed in monocytes, granulocytes (PubMed:1346576), and neutrophils (PubMed:21193407); localized to the cell membrane and membrane rafts. Related references: PMID:1346576, PMID:21193407
• Genetic variants of ITGAM are associated with systemic lupus erythematosus (SLEB6).

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide derived from human CD11b; consider epitope mapping to confirm recognition site if specific domain binding is required.
• For flow cytometry, titrate the antibody using relevant human cell populations (e.g., peripheral blood monocytes, granulocytes, or cell lines like THP-1) to determine optimal staining concentration.
• Use Fc receptor blocking reagents to minimize non-specific binding to Fcγ receptors expressed on myeloid cells.
• Include an appropriate APC-conjugated isotype control and perform compensation controls when using in multicolor panels.
• Verify target expression by co-staining with a non-competing CD11b antibody clone or using a knockout/knockdown control where feasible.
• The predicted molecular weight of ITGAM is approximately 127 kDa; if adapting this antibody for other applications (e.g., Western blot), note that it may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, technical services, and consulting from concept to clinic. This APC-conjugated anti-CD11b monoclonal antibody is suitable for innate immunity, inflammation, and leukocyte adhesion research, and can be complemented by our offering of validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For custom development or sourcing of rare-target reagents, our dedicated team provides scalable, reliable solutions to accelerate your assay development and production.