APC-conjugated Rabbit anti-Mouse Foxp3 Monoclonal Antibody for Flow Cytometry (Intracellular) - Q99JB6

Core Product Specifications and Parameters

Biological Background: Foxp3 Function and Localization

• Foxp3 (Forkhead box protein P3), also known as Scurfin, is a transcription factor of the forkhead/winged-helix family.
• It is a master regulator for the development and suppressive function of regulatory T-cells (Treg), essential for immune homeostasis. Related references: PMID:22813742
• Foxp3 can function as a transcriptional repressor or activator, interacting with histone acetylases/deacetylases and other transcription factors to modulate gene expression. Related references: PMID:15790681 PMID:17377532
• It represses cytokines like IL2 and IFNG, and activates genes such as CTLA4 and TNFRSF18, shaping Treg suppressive activity. Related references: PMID:17377532 PMID:19696312
• Subcellularly, Foxp3 is found in both the nucleus and cytoplasm, consistent with its roles in transcription regulation.
• It undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which regulate its activity and stability.
• High expression of Foxp3 is observed in thymus and spleen, tissues central to T-cell development and function.
• As a zinc-finger protein, Foxp3 binds DNA and metal ions, contributing to its structural and functional properties.

Experimental Guidance and Technical Tips

• The immunogen targets an N-terminal peptide (aa 1-100) of mouse Foxp3; consider verifying specificity for full-length protein variants in your relevant cell system.
• For intracellular flow cytometry, optimize fixation and permeabilization protocols to ensure robust detection of nuclear Foxp3.
• This monoclonal antibody is validated for mouse reactivity only; cross-reactivity with other species has not been tested; include appropriate species-specific controls.
• The APC conjugate allows direct detection; verify detector compatibility and perform compensation as needed.
• Foxp3 is a hallmark of Treg cells; use known markers (e.g., CD4+CD25+) as positive controls and validate in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to high-quality IVD raw materials, including this APC-conjugated anti-Foxp3 antibody for Treg-focused immunoassays. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials—all designed to support your workflow from concept to clinic. Contact our team for customized procurement solutions tailored to your development needs.