APC-conjugated Rabbit anti-Human CD62L/L-Selectin Monoclonal Antibody for Flow Cytometry - P14151

Core Product Specifications and Parameters

Biological Background: L-selectin Function and Localization

• L-selectin (CD62L, SELL) is a calcium-dependent (C-type) lectin that mediates cell adhesion by binding to glycoprotein ligands on adjacent cells, facilitating lymphocyte homing to peripheral lymph nodes and leukocyte tethering/rolling on endothelia.
  Related references:
  PMID:12403782 PMID:28011641 PMID:28489325
• Encoded by the SELL gene, L-selectin is a type I transmembrane protein belonging to the selectin family, characterized by an N-terminal C-type lectin domain, an epidermal growth factor (EGF)-like domain, and short consensus repeat (sushi) domains.
• It is expressed on the surface of most peripheral blood B cells and T lymphocytes, as well as on other leukocytes.
• L-selectin localizes to the cell membrane, consistent with its role in cell adhesion and leukocyte trafficking.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation; its domain architecture features an EGF-like and sushi repeats, as classified by UniProt keywords.
• Alternative initiation may generate isoforms with distinct N-termini.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 39–332) of human L-selectin, suggesting the antibody is suitable for detecting cell-surface L-selectin on live cells without permeabilization in flow cytometry.
• For flow cytometry (FC), optimize the antibody concentration by titration and include appropriate isotype controls.
• Validate staining using human peripheral blood mononuclear cells (PBMCs) or L-selectin-positive cell lines; co-staining with lineage markers (e.g., CD3, CD19) can help analyze lymphocyte subsets.
• If necessary, consider combining this antibody with other fluorophore-conjugated antibodies for multicolor flow cytometry panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing one-stop access to validated monoclonal and polyclonal antibodies, recombinant proteins, bulk ancillary reagents, and custom sourcing services. This anti-L-selectin antibody supports research in immunology and cell adhesion, covering key areas of leukocyte biology. Our integrated supply chain and technical consultancy help streamline your assay development from concept to clinic.