APC-conjugated Rabbit anti-Human CD3E Monoclonal Antibody for Intracellular Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• CD3E is the epsilon chain of the T-cell surface glycoprotein CD3, also known as T3E, TCRE, IMD18, or CD3epsilon.
• It is a core component of the TCR-CD3 complex on T lymphocytes, playing an essential role in adaptive immune response. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• Phosphorylation of CD3E ITAMs by LCK and FYN upon TCR engagement leads to downstream signaling activation. Related references: PMID:2470098, PMID:40592325
• CD3E is essential for proper T-cell development.
• CD3E mediates internalization and surface down-regulation of TCR-CD3 complexes through endocytosis sequences in its cytosolic region. Related references: PMID:10384095, PMID:26507128
• CD3E functions as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 binding and blocking CD3E-ZAP70 interaction. Related references: PMID:38614099, PMID:12110186
• CD3E is a single-pass type I membrane protein localized to the cell membrane.
• Post-translational modifications include phosphorylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody was raised against a synthetic peptide covering amino acids 50-150, which lies within the extracellular domain of CD3E. It is recommended for intracellular flow cytometry; ensure proper permeabilization and fixation protocols to access the cytoplasmic epitope.
• Validate the antibody in your specific experimental system, as performance may vary across different T-cell subsets or activation states.
• Consider using appropriate isotype controls and titrating the antibody to optimize signal-to-noise ratio in intracellular staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD3E-targeted research in T-cell immunology, we offer tailored sourcing solutions to accelerate your assay development from concept to clinic. Our expertise ensures you receive consistent, high-quality materials even for rare targets.