APC-conjugated Rabbit anti-Human CD301/CLEC10A Monoclonal Antibody for Flow Cytometry - Q8IUN9

Core Product Specifications and Parameters

Biological Background: CD301/CLEC10A Function and Localization

• CLEC10A (C-type lectin domain family 10 member A), also known as macrophage galactose-type lectin (MGL), DC-ASGPR, or CD301, is a type II transmembrane protein belonging to the C-type lectin family. It is encoded by the CLEC10A gene on chromosome 17 and exists in several alternatively spliced isoforms.

• Binds in a Ca²⁺-dependent manner to terminal N-acetylgalactosamine (GalNAc) residues, including Tn antigen, T antigen, LacdiNAc, and GM2/GD2 gangliosides, acting as a pattern recognition receptor on myeloid antigen presenting cells. Related references: PMID:15802303, PMID:23507963

• Functions as a signaling receptor at the APC–T cell interface: on immature dendritic cells, engagement with Tn antigen on CD45 receptor modulates T-cell activation thresholds, cytokine production, and proliferation, and promotes a tolerogenic dendritic cell phenotype that induces regulatory T cells. Related references: PMID:15802303, PMID:16998493, PMID:22213806

• Serves as an endocytic receptor that internalizes Tn-antigen–bearing tumor-associated mucins, facilitating cross-presentation to CD8⁺ and CD4⁺ T cells and contributing to anti-tumor immunity. Related references: PMID:15802303, PMID:17616966, PMID:17804752

• Subcellular localization: predominantly at the cell membrane; upon ligand binding, internalized to early endosomes and lysosomes.

• Tissue specificity: expressed in myeloid antigen presenting cells in lymph nodes and skin, including dermal dendritic cells (confirmed at protein level).

• Contains a single C-type lectin domain, an N-terminal cytoplasmic tail, a transmembrane helix, and a coiled-coil domain. Post-translational modifications include N-linked glycosylation, and the protein forms disulfide-linked homodimers. Multiple isoforms arise from alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular C-type lectin domain (aa 61–316), and the antibody is directly conjugated to APC, making it suitable for surface staining in flow cytometry. Use appropriate isotype controls and Fc receptor blocking when analyzing human samples.

• CD301/CLEC10A is primarily expressed on dendritic cells and macrophages; consider co-staining with lineage markers (e.g., CD11c, CD14) for accurate population identification.

• Reported molecular weights of 28, 32, and 35 kDa likely reflect differential glycosylation or alternative splice isoforms; while this product is validated for flow cytometry, these weight variants may be observed in western blot under reducing conditions if tested experimentally.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized recombinant proteins, and ancillary reagents. For researchers and diagnostic developers working in immunology and dendritic cell biology, we supply high-quality monoclonal antibodies like this CLEC10A clone, along with technical support and bulk sourcing options. Our one-stop model accelerates development from early research to clinical application.