APC-conjugated Anti-Siglec-7/CD328 Monoclonal Antibody (Rabbit) for Flow Cytometry - Q9Y286

Core Product Specifications and Parameters

Biological Background: Siglec-7/CD328 Function and Localization

• Siglec-7 (Sialic acid-binding Ig-like lectin 7), also known as CD328, AIRM-1, p75, or D-siglec, is a type I transmembrane protein belonging to the immunoglobulin superfamily and siglec family of sialic acid-binding lectins.
• Functions as an inhibitory receptor on immune cells, preferentially binding α-2,3- and α-2,6-linked sialic acids on target cells; ligand engagement triggers tyrosine phosphorylation of its ITIM motifs, recruiting SH2-domain-containing phosphatases to block activating signals.
• Plays a key role in regulating natural killer (NK) cell cytotoxicity, inhibiting killing of sialic acid-expressing targets; also suppresses differentiation of CD34+ hematopoietic precursors toward the myelomonocytic lineage and inhibits proliferation of leukemic myeloid cells.
• Expressed predominantly on resting and activated NK cells, and at lower levels on granulocytes and monocytes; high expression observed in placenta, liver, lung, spleen, and peripheral blood leukocytes.
• Localized to the plasma membrane as a single-pass transmembrane protein; extracellular region contains Ig-like C2-type domains responsible for sialic acid recognition, which may be masked by cis interactions on the same cell surface.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, with the phosphotyrosine sites serving as docking sites for SH2-domain proteins.
• Alternative splicing generates multiple transcript variants, potentially modulating ligand-binding properties or expression levels.
• Structural studies (3D-structure available) reveal the molecular basis for sialic acid specificity, aiding in understanding its immunomodulatory functions.

Experimental Guidance and Technical Tips

• This APC-conjugated antibody (clone not specified) was raised against the extracellular domain (aa 18-144) of human Siglec-7, making it suitable for detecting cell surface expression by flow cytometry.
• For FC, titrate the antibody to determine optimal concentration; use a matched isotype control to set gates and assess background.
• Validate staining in known positive cell lines or primary human NK cells; cross-reactivity is reported for human only, so ensure the experimental system involves human samples.
• Consider that Siglec-7 may undergo cis-interactions masking epitopes; pretreatment with sialidase may enhance detection in some cell types.
• This product is provided as an APC conjugate; handle with care to avoid photobleaching, and use appropriate compensation controls in multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets. For Siglec-7/CD328 research, we support the development of immune checkpoint diagnostics and NK cell-based assays with high-quality reagents like this APC-anti-Siglec-7 mAb, alongside technical consultation from concept to clinic. Let CamelBio bridge your sourcing needs with reliable, scalable solutions.