APC-conjugated Anti-CD223/LAG-3 Rabbit Monoclonal Antibody for Flow Cytometry - P18627

Core Product Specifications and Parameters

Biological Background: LAG3 Function and Localization

• Encoded by the LAG3 gene, lymphocyte activation gene 3 protein (also known as CD223) is a type I transmembrane protein of the immunoglobulin superfamily with a molecular weight of approximately 57 kDa.
• Acts as an inhibitory receptor on antigen-activated T-cells, delivering negative signals upon binding to its main ligand MHC class II on antigen-presenting cells or FGL1, thereby inhibiting T-cell receptor (TCR) signaling and T-cell activation. Related references: PMID:20421648, PMID:35761082, PMID:7805750, PMID:30580966, PMID:32920841
• Ligand binding induces ubiquitination of the KIEELE motif, exposing the RRFSALE motif and promoting formation of condensates with the TCR component CD3E, which disrupts the CD3E-LCK association and blocks TCR activation. Related references: PMID:40101708, PMID:40592325
• Negatively regulates the proliferation, activation, effector function, and homeostasis of both CD4+ and CD8+ T-cells, and contributes to the suppressive function of regulatory T-cells (Tregs) as well as regulation of plasmacytoid dendritic cells. Related references: PMID:20421648, PMID:7805750, PMID:8647185
• The LAG3-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity; co-blockade of LAG3 and PD-1 reverses T-cell exhaustion and normalizes the anti-tumor response, providing a rationale for cancer immunotherapy. Related references: PMID:35981087, PMID:40101708
• May also function as a ligand for MHC class II on antigen-presenting cells, promoting their activation and driving Th1 immune responses.
• Subcellular localization: primarily at the cell membrane; a secreted form also exists. Post-translational modifications include disulfide bonds, glycosylation, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-430) of human LAG3, suggesting the antibody recognizes an epitope accessible on the cell surface; ideally suited for flow cytometry analysis of live cells.
• As the antibody is directly conjugated to APC, it enables one-step staining without a secondary reagent, reducing background and simplifying the workflow.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions. Include an appropriate isotype control to assess non-specific binding.
• Verify expression in activated T-cell populations or LAG3-positive cell lines. Surface staining may require fresh, unfixed cells; if fixation is used, check compatibility with the APC fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This product targets LAG3, a key immune checkpoint receptor, supporting immuno-oncology research and assay development. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow and accelerate development.